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Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities

Abstract : Exopolysaccharides (EPS) from cell-free Porphyridiwn cruentum media were concentrated then purified (diafiltration) on a 0.14 ism ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L h(-1). m(-2) were obtained during the concentration at 4 bars for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m s(-1); 49.7 Lm(-1). M-2 for the diafiltration at 3.3 m s(-1). Permeate fluxes were correctly predicted from polysaccharide concentrations (10% deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or time of filtration could be modulated following the cross-flow velocity. Polysaccharides were concentrated 6.3 to 10.4 times in such a way that the final sugars concentration reached 1.74-2.26 g L-1. Rheological behavior of filtered solutions changed following the concentration progress. More than 80% (w/w) of polysaccharides were recovered while 49% and 99% of proteins and salts were removed respectively. The filtrations allowed reaching a final monosaccharide content of dry matter equal to 48.9% against 0.6% (w/w) initially.
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https://hal.archives-ouvertes.fr/hal-01925710
Contributor : Anthony Massé <>
Submitted on : Saturday, November 17, 2018 - 12:17:25 PM
Last modification on : Wednesday, March 31, 2021 - 3:37:18 AM

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Rafik Balti, Romain Le Balc’h, Nicolas Brodu, Marthe Gilbert, Benjamin Le Gouic, et al.. Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities. Process Biochemistry, Elsevier, 2018, 74, pp.175 - 184. ⟨10.1016/j.procbio.2018.06.021⟩. ⟨hal-01925710⟩

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