Phosphoinositides regulate the TCR/CD3 complex membrane dynamics and activation

Nassima Chouaki-Benmansour (1) , Kilian Ruminski (1) , Anne-Marie Sartre (1) , Marie-Claire Phelipot (1) , Audrey Salles (1) , Elise Bergot (1) , Ambroise Wu , Gaëtan Chicanne (2) , Mathieu Fallet (1) , Sophie Brustlein (3) , Cyrille Billaudeau (4) , Anthony Formisano , Sébastien Mailfert (1) , Bernard Payrastre (5) , Didier Marguet (1) , Sophie Brasselet (3) , Yannick Hamon , Hai-Tao He (1)

1 CIML - Centre d'Immunologie de Marseille - Luminy
2 I2MC - Institut des Maladies Métaboliques et Cardiovasculaires
3 FRESNEL - Institut FRESNEL
4 LPN - Laboratoire de photonique et de nanostructures
5 CPTP - Centre de Physiopathologie Toulouse Purpan

Phosphoinositides (PIs) play important roles in numerous membrane-based cellular activities. However, their involvement in the mechanism of T cell receptor (TCR) signal transduction across the plasma membrane (PM) is poorly defined. Here, we investigate their role, and in particular that of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] in TCR PM dynamics and activity in a mouse T-cell hybridoma upon ectopic expression of a PM-localized inositol polyphosphate-5-phosphatase (Inp54p). We observed that dephosphorylation of PI(4,5)P2 by the phosphatase increased the TCR/CD3 complex PM lateral mobility prior stimulation. The constitutive and antigen-elicited CD3 phosphorylation as well as the antigen-stimulated early signaling pathways were all found to be significantly augmented in cells expressing the phosphatase. Using state-of-the-art biophotonic approaches, we further showed that PI(4,5)P2 dephosphorylation strongly promoted the CD3ε cytoplasmic domain unbinding from the PM inner leaflet in living cells, thus resulting in an increased CD3 availability for interactions with Lck kinase. This could significantly account for the observed effects of PI(4,5)P2 dephosphorylation on the CD3 phosphorylation. Our data thus suggest that PIs play a key role in the regulation of the TCR/CD3 complex dynamics and activation at the PM.

Domaines: Optique / photonique

Nassima Chouaki-Benmansour, Kilian Ruminski, Anne-Marie Sartre, Marie-Claire Phelipot, Audrey Salles, et al.. Phosphoinositides regulate the TCR/CD3 complex membrane dynamics and activation. Scientific Reports, 2018, 8 (1), pp.4966. ⟨10.1038/s41598-018-23109-8⟩. ⟨hal-01920092⟩