A simple method for the separation of Bacillus thuringiensis spores and crystals

Jihane Rahbani (1, 2) , Dominique Salameh (2) , Mireille Kallassy Awad , Laure Chamy , Cédric Brandam (1) , Roger Lteif (2)

1 LGC - Laboratoire de Génie Chimique
2 USJ - Université Saint-Joseph de Beyrouth

A simple new method, for separating Bacillus thuringiensis crystals from spores and cell debris, is described. The developed purification method uses hexane and low speed centrifugation and does not require any expensive material or reagents.

Mots clés: Bacillus thuringiensis; Crystals; Hexane; Purification; Spores

Domaines: Génie chimique; Génie des procédés

Jihane Rahbani, Dominique Salameh, Mireille Kallassy Awad, Laure Chamy, Cédric Brandam, et al.. A simple method for the separation of Bacillus thuringiensis spores and crystals. Journal of Microbiological Methods, 2014, 107, pp.147-149. ⟨10.1016/j.mimet.2014.10.003⟩. ⟨hal-01919681⟩