Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy.

Serena Flori (1) , Pierre-Henri Jouneau (2) , Benoit Gallet (3) , Leandro F Estrozi (3) , Christine Moriscot (3) , Guy Schoehn (3) , Giovanni Finazzi (1) , Denis Falconet (1)

1 LPCV - Physiologie cellulaire et végétale
2 LEMMA - Laboratoire d'Etude des Matériaux par Microscopie Avancée
3 IBS - UMR 5075 - Institut de biologie structurale

Internal chloroplast structures present complex and various characteristics, which are still largely undetermined due to insufficient imaging investigation. Information on chloroplast morphology has traditionally been collected using light microscopy (LM), confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) techniques. However, recent technological progresses in the field of microscopy have made it possible to visualize the internal structure of chloroplast in far greater detail and in 3D. Here we recapitulate protocols to visualize chloroplasts from Arabidopsis leaves and Phaeodactylum tricornutum cells with confocal and transmission electron microscopy together with a new technique using a focused ion beam-scanning electron microscope (FIB-SEM) allowing for 3D imaging.

Mots clés: Tomography; Confocal imaging; Arabidopsis; Focused ion beam (FIB); Phaeodactylum tricornutum; Scanning electron microscopy (SEM); Transmission electron microscopy (TEM)

Domaines: Biologie structurale [q-bio.BM]; Biochimie, Biologie Moléculaire

Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F Estrozi, Christine Moriscot, et al.. Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy.. Marechal Eric. Plastids: Methods and Protocols. Series: Methods in Molecular Biology, 1829, Springer, pp.113-122, 2018, Methods in Molecular Biology, 978-1-4939-8654-5; 978-1-4939-8653-8. ⟨10.1007/978-1-4939-8654-5_7⟩. ⟨hal-01913145⟩