Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A

Rachelle El Khoury (1, 2) , Florence Mathieu (1) , Ali Atoui (3) , Hiba Kawtharani (2) , Anthony El Khoury (2) , Charbel Afif (2) , Richard Maroun (2) , André El Khoury (2)

1 LGC - Laboratoire de Génie Chimique
2 USJ - Université Saint-Joseph de Beyrouth
3 LU / ULB - الجامعة اللبنانية [بيروت] = Lebanese University [Beirut] = Université libanaise [Beyrouth]

Ochratoxin A (OTA) is one of the most important mycotoxins, and contaminates several agricultural products, particularly cereals, grapes, maize, barley, spices and coffee. The aim of this project was to reduce the levels of OTA by supplementing the artificially contaminated solutions with seven strains of actinobacteria (AT10, AT8, SN7, MS1, ML5, G10 and PT1) in order to evaluate their capacity for binding and metabolizing the OTA, as well as their ability to reduce the expression of the genes responsible for its production in A. carbonarius. In the first part of this study, we evaluated the capacity of Streptomyces strains for binding OTA on their surfaces after 0, 30 and 60 min of incubation with PBS solution supplemented with OTA. In the second part, we tested the ability of these strains, as well as their supernatants, to detoxify the ISP2 medium. Finally, we studied the effect of the Streptomyces cocultured with Aspergillus carbonarius on the expression of OTA biosynthesis genes. Results showed that, among the strains co-cultured with A. carbonarius, the strain G10 was able to reduce the expression of acpks, acOTApks, acOTAnrps and vea genes, thus reducing OTA from solid PDA medium to 13.50% of reduction. This strain was remarkably able to detoxify and bind OTA up to 47.07%. Strain AT8 was stronger in detoxifying OTA (52.61%), but had no significant effect on the studied gene expression.

Mots clés: Ochratoxin A; Actinobacteria; Binding; Biocontrol; Detoxification; Genes expression

Domaines: Bactériologie; Biotechnologies

Rachelle El Khoury, Florence Mathieu, Ali Atoui, Hiba Kawtharani, Anthony El Khoury, et al.. Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A. Toxins, 2017, 9 (7), pp.222. ⟨10.3390/toxins9070222⟩. ⟨hal-01896324⟩