Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition

Haohao Duan (1, 2, 3) , Mark Donovan (1) , Aude Foucher (1) , Xavier Schultze (1) , Sébastien Lecommandoux (2, 3)

1 L'Oréal Recherche - L'Oréal Recherche France
2 LCPO - Laboratoire de Chimie des Polymères Organiques
3 Team 3 LCPO : Polymer Self-Assembly & Life Sciences

Polysaccharides represent a versatile class of building blocks that are used in macromolecular design. By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures. Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM). The copolymers could self-assemble to form particles in water by nanoprecipitation. In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers. By controlling the self-assembly process, five particle samples with different morphologies and compositions were developed. The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR). We demonstrated that the particle-protein interaction could be modulated by the particle structure and composition. It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.

Domaines: Polymères; Chimie thérapeutique

Haohao Duan, Mark Donovan, Aude Foucher, Xavier Schultze, Sébastien Lecommandoux. Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition. Scientific Reports, 2018, 8 (1), pp.14730. ⟨10.1038/s41598-018-32994-y⟩. ⟨hal-01893210⟩