Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition

Abstract : Polysaccharides represent a versatile class of building blocks that are used in macromolecular design. By choosing the appropriate saccharide block, various physico-chemical and biological properties can be introduced both at the level of the polymer chains and the resulting self-assembled nanostructures. Here, we synthetized amphiphilic diblock copolymers combining a hydrophobic and helical poly(γ-benzyl-L-glutamate) PBLG and two polysaccharides, namely hyaluronic acid (HA) and laminarin (LAM). The copolymers could self-assemble to form particles in water by nanoprecipitation. In addition, hybrid particles containing both HA and LAM in different ratios were obtained by co-nanoprecipitation of the two copolymers. By controlling the self-assembly process, five particle samples with different morphologies and compositions were developed. The interaction between the particles and biologically relevant proteins for HA and LAM, namely CD44 and Dectin-1 respectively, was evaluated by surface plasmon resonance (SPR). We demonstrated that the particle-protein interaction could be modulated by the particle structure and composition. It is therefore suggested that this method based on nanoprecipitation is a practical and versatile way to obtain particles with controllable interactions with proteins, hence with the appropriate biological properties for biomedical applications such as drug delivery.
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Submitted on : Thursday, October 11, 2018 - 11:08:58 AM
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Haohao Duan, Mark Donovan, Aude Foucher, Xavier Schultze, Sébastien Lecommandoux. Multivalent and multifunctional polysaccharide-based particles for controlled receptor recognition. Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.14730. ⟨10.1038/s41598-018-32994-y⟩. ⟨hal-01893210⟩

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