Streptomonospora algeriensis sp. nov., a halophilic actinomycete isolated from soil in Algeria

Atika Meklat (1, 2) , Noureddine Bouras (2) , Amar Riba (2) , Abdelghani Zitouni (2) , Florence Mathieu (3) , Manfred Rohde (4) , Peter Schumann (5) , Cathrin Spröer (5) , Hans-Peter Klenk (5) , Nasserdine Sabaou (2)

1 UB1 - Université Saâd Dahlab Blida 1
2 ENS Kouba-Alger - École normale supérieure - Kouba-Alger
3 LGC - Laboratoire de Génie Chimique
4 HZI - Helmholtz Centre for Infection Research
5 DSMZ - Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH / Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures

A halophilic actinomycete strain, designated H27T, was isolated from a soil sample collected from a hypersaline habitat in Djelfa Province (North-Central Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce poor aerial mycelium, which formed short chains of oval to cylindrical-shaped spores at maturity, and non fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 10–15 % (w/v) and the optimum growth temperature and pH were found to be 28–37 °C and 6–7, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain H27T were identified as MK-11 (H4) and MK-10 (H6). The major fatty acids were found to be iso-C16:0, anteiso-C17:0, 10 methyl C17:0 and 10 methyl C16:0. The diagnostic phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The chemotaxonomic properties of strain H27T are consistent with those shared by members of the genus Streptomonospora. 16S rRNA gene sequence analysis indicated that strain H27T is most closely related to Streptomonospora alba DSM 44588T (98.8 %) and Streptomonospora flavalba DSM 45155T (98.7 %) whereas the DNA–DNA relatedness values between strain H27T and the two type strains were 17.1 and 57.9 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain H27T should be classified as representative of a novel species, for which the name Streptomonospora algeriensis sp. nov. is proposed. The type strain is H27T (=DSM 45604T =CCUG 63369T =MTCC 11563T).

Mots clés: Halophilic actinomycete; Streptomonosporaalgeriensis sp. nov; Streptomonospora; Polyphasic taxonomy; Algerian soil

Domaines: Systématique, phylogénie et taxonomie; Bactériologie

Atika Meklat, Noureddine Bouras, Amar Riba, Abdelghani Zitouni, Florence Mathieu, et al.. Streptomonospora algeriensis sp. nov., a halophilic actinomycete isolated from soil in Algeria. Antonie van Leeuwenhoek, 2014, 106 (2), pp.287-292. ⟨10.1007/s10482-014-0195-3⟩. ⟨hal-01893097⟩