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Identification of Two Putative Acyltransferase Genes Potentially Implicated in Dithiolopyrrolone Biosyntheses in Saccharothrix algeriensis NRRL B-24137

Abstract : The dithiolopyrrolone class of antibiotics has been known to display bacteriostatic activity against both Gram-positive and Gram-negative bacteria and exert other biological activities. Acyltransferase activities are proposed to be responsible for the structural diversity of dithiolopyrrolones produced by Saccharothrix algeriensis NRRL B-24137. Moreover, two activities, pyrrothine N-acetyltransferase and pyrrothine N-benzoyltransferase, are reported to catalyze the formation, respectively, to thiolutin and benzoyl-pyrrothine (BEP) in this bacterium. In this study, two genes encoding two putative acyltransferases were identified in S. algeriensis. The first one, actA, was identified by bioinformatic analysis and by analogy to an acetyltransferase, hlmA, identified in holomycin biosynthetic gene cluster in Streptomyces clavuligerus. The second was identified by purification of both enzymes from the bacterial biomass which provided a semipurified extract. The microsequencing of tryptic peptides from the final protein preparation yielded sequences of eight different fragments, two of them encoded by one gene, actB, in S. algeriensis genome bank. The alignment of actB against the GenBank database revealed significant homology to acyltransferase family. Differential expression of these genes, actA and actB, was then investigated in three different media: (i) semisynthetic medium (SSM), which promotes the production of thiolutin; (ii) SSM supplemented by 1.25 mM benzoic acid (SSM + BA), which promotes the production of both thiolutin and BEP; and (iii) tryptic soy broth (TSB) in which no dithiolopyrrolone derivatives were detected.
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Safwan Saker, Ahmed Lebrihi, Florence Mathieu. Identification of Two Putative Acyltransferase Genes Potentially Implicated in Dithiolopyrrolone Biosyntheses in Saccharothrix algeriensis NRRL B-24137. Applied Biochemistry and Biotechnology, Humana Press, 2014, 173 (3), pp.787-802. ⟨10.1007/s12010-014-0896-0⟩. ⟨hal-01890816⟩

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