Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

Sylvie Zida (1, 2) , Edouard Tuaillon (1, 2) , Makoura Barro (3) , Arnaud Kwimatouo Lekpa Franchard (4) , Thérèse Kagoné (4) , Boubacar Nacro (3) , Abdoul Salam Ouedraogo (5) , Karine Bolloré (1, 2) , Armen Sanosyan (1, 2) , Jean-Christophe Plantier (6) , Nicolas Meda (4) , Lassana Sangare (5) , Christine Rouzioux (7) , François Rouet (8) , Dramane Kania (4)

1 Pathogénèse et contrôle des infections chroniques (PCCI)
2 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
3 CHUSS - Centre Hospitalier Universitaire Souro Sanou [Bobo-Dioulasso]
4 Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz
5 CHUYO - Centre Hospitalier Universitaire Yalgado Ouédraogo
6 Laboratoire de virologie [Rouen]
7 UPD5 - Université Paris Descartes - Paris 5
8 Institut Pasteur du Cambodge

The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/10(6) peripheral blood mononuclear cells.

Domaines: Microbiologie et Parasitologie

Sylvie Zida, Edouard Tuaillon, Makoura Barro, Arnaud Kwimatouo Lekpa Franchard, Thérèse Kagoné, et al.. Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients. Journal of Clinical Microbiology, 2016, 54 (6), pp.1641 - 1643. ⟨10.1128/JCM.03372-15⟩. ⟨hal-01872543⟩