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Exploring the microbiome of the “star” freshwater diatom Asterionella formosa in a laboratory context

Kojadinovic-Sirinelli Mila 1, 2 Villain Adrien 3 Puppo Carine 1 Fon Sing Sophie 1 Prioretti Laura 1 Hubert Pierre 4 Gérald Grégori 5 Zhang Yizhi 1 Sassi Jean-François 6 Claverie Jean-Michel 3 Guillaume Blanc 5 Brigitte Gontero 1
1 BIP - Bioénergétique et Ingénierie des Protéines
2 MEM - Microbiologie Environnementale et Moléculaire
BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM
3 IGS - Information génomique et structurale
4 LISM - Laboratoire d'Ingéniérie et Science des Matériaux - EA 4695
5 MIO - Institut méditerranéen d'océanologie
6 CEA Cadarache
Abstract : Most of our knowledge on the mechanisms underlying diatom-bacterial interactions has been acquired through studies involving isolation of culturable partners. Here, we established a laboratory model of intermediate complexity between complex natural communities and laboratory pure culture models. We investigated the whole community formed by the freshwater diatom Asterionella formosa and its associated bacteria in a laboratory context, including both culturable and unculturable bacteria. Combining cellular and molecular approaches, we showed that in laboratory cultures, A. formosa microbiome was dynamic and comprised of numerous bacterial species (mainly Proteobacteria and Bacteroidetes). Using metagenomics, we explored several metabolic potentials present within the bacterial community. Our analyses suggested that bacteria were heterotrophic although a third of them (Alpha- and Beta-proteobacteria) could also be phototrophic. About 60% of the bacteria, phylogenetically diverse, could metabolize glycolate. The capacity to synthesize molecules such as B vitamins appeared unevenly distributed among bacteria. Altogether, our results brought insights into the bacterial diversity found in diatom-bacterial communities and hinted at metabolic interdependencies within the community that could result in diatom-bacterial and bacterial-bacterial interactions. The present work allowed us to explore the functional architecture of the bacterial community associated with A. formosa in culture and is complementary to field studies. This article is protected by copyright. All rights reserved.
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https://hal-amu.archives-ouvertes.fr/hal-01863990
Contributor : Olivier Poirot <>
Submitted on : Wednesday, August 29, 2018 - 11:52:12 AM
Last modification on : Monday, May 17, 2021 - 2:52:22 PM

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UNIV-AMU | CNRS | UNIV-TLN | MIO | OSU-INSTITUT-PYTHEAS | CEA | LISM | URCA | CEA-CAD | CEA-DRF | IGS | INSU | MIO-MEB | MIO-CYBELE | BIAM

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Kojadinovic-Sirinelli Mila, Villain Adrien, Puppo Carine, Fon Sing Sophie, Prioretti Laura, et al.. Exploring the microbiome of the “star” freshwater diatom Asterionella formosa in a laboratory context. Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2018, 20 (10), pp.3601-3615. ⟨10.1111/1462-2920.14337⟩. ⟨hal-01863990⟩

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