 |
Journal articles
Exploring the microbiome of the “star” freshwater diatom Asterionella formosa in a laboratory context
Kojadinovic-Sirinelli Mila
1, 2
Villain Adrien
3
Puppo Carine
1
Fon Sing Sophie
1
Prioretti Laura
1
Hubert Pierre
4
Gérald Grégori
5
Zhang Yizhi
1
Sassi Jean-François
6
Claverie Jean-Michel
3
Guillaume Blanc
5
Gontero Brigitte
1
2
MEM - Microbiologie Environnementale et Moléculaire
BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM
Kojadinovic-Sirinelli Mila 1, 2
Author
Villain Adrien 3
Author
Puppo Carine 1
Author
Fon Sing Sophie 1
Author
Prioretti Laura 1
Author
Hubert Pierre 4
Author
Gérald Grégori 5
Mail :
Author IdHAL : gerald-gregori ResearcherId : http://www.researcherid.com/rid/D-8657-2013 ORCID : https://orcid.org/0000-0003-1645-9468
Zhang Yizhi 1
Author
Sassi Jean-François 6
Author
Claverie Jean-Michel 3
Author
Guillaume Blanc 5
Mail :
Author IdHAL : guillaume-blanc ORCID : https://orcid.org/0000-0001-5728-1104
Gontero Brigitte 1
Author
1
BIP - Bioénergétique et Ingénierie des Protéines (CNRS - Université d’ Aix-Marseille
31, chemin Joseph Aiguier
13402 MARSEILLE Cedex 20 FRANCE
Tél : +33 (0)4 91 16 45 50 - France)
- AMU - Aix Marseille Université : UMR7281 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7281 (France)
2
MEM - Microbiologie Environnementale et Moléculaire (ex-LBC (Laboratoire de Bioénergétique Cellulaire) - France)
- BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM (Anciennement "Institut de Biologie Environnementale et Biotechnologie (IBEB)"
Centre CEA Cadarache
13108 Saint Paul lez Durance
- France)
- CNRS - Centre National de la Recherche Scientifique : UMR 7265 (France)
- AMU - Aix Marseille Université : UMR 7265 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/BIAM (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
3
IGS - Information génomique et structurale (Parc Scientifique de Luminy
163 Avenue de Luminy
Case 934
13288 Marseille Cedex 09 - France)
- AMU - Aix Marseille Université : UMR7256 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
- CNRS - Centre National de la Recherche Scientifique : UPR2589 (France)
4
LISM - Laboratoire d'Ingéniérie et Science des Matériaux - EA 4695 (Université de Reims Champagne-Ardenne
UFR Sciences Exactes et Naturelles
Campus Moulin de la Housse
BP 1039
51687 REIMS CEDEX 2 - France)
- URCA - Université de Reims Champagne-Ardenne : EA 4695 (9 boulevard de la Paix - CS 60005 - 51724 Reims cedex - France)
- SFR Condorcet (France)
- URCA - Université de Reims Champagne-Ardenne : CNRS 3417 (9 boulevard de la Paix - CS 60005 - 51724 Reims cedex - France)
- UPJV - Université de Picardie Jules Verne : CNRS 3417 (Chemin du Thil - 80000 Amiens - France)
- CNRS - Centre National de la Recherche Scientifique : CNRS 3417 (France)
5
MIO - Institut méditerranéen d'océanologie (M.I.O. Institut Méditerranéen d'Océanologie
Bâtiment Méditerranée
163 Avenue de Luminy
13288 Marseille - France)
- IRD - Institut de Recherche pour le Développement : UMR_D235 (Siège Le Sextant 44, bd de Dunkerque CS 90009 13572 Marseille cedex 02 - France)
- AMU - Aix Marseille Université : UM110 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
- UTLN - Université de Toulon : UMR7294 (Avenue de l'Université - BP 20132 - 83957 La Garde Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7294 (France)
6
CEA Cadarache (13108 Saint-Paul-les-Durance Cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
Abstract : Most of our knowledge on the mechanisms underlying diatom-bacterial interactions has been acquired through studies involving isolation of culturable partners. Here, we established a laboratory model of intermediate complexity between complex natural communities and laboratory pure culture models. We investigated the whole community formed by the freshwater diatom Asterionella formosa and its associated bacteria in a laboratory context, including both culturable and unculturable bacteria. Combining cellular and molecular approaches, we showed that in laboratory cultures, A. formosa microbiome was dynamic and comprised of numerous bacterial species (mainly Proteobacteria and Bacteroidetes). Using metagenomics, we explored several metabolic potentials present within the bacterial community. Our analyses suggested that bacteria were heterotrophic although a third of them (Alpha- and Beta-proteobacteria) could also be phototrophic. About 60% of the bacteria, phylogenetically diverse, could metabolize glycolate. The capacity to synthesize molecules such as B vitamins appeared unevenly distributed among bacteria. Altogether, our results brought insights into the bacterial diversity found in diatom-bacterial communities and hinted at metabolic interdependencies within the community that could result in diatom-bacterial and bacterial-bacterial interactions. The present work allowed us to explore the functional architecture of the bacterial community associated with A. formosa in culture and is complementary to field studies. This article is protected by copyright. All rights reserved.
Document type :
Journal articles
Complete list of metadatas
https://hal-amu.archives-ouvertes.fr/hal-01863990
Contributor : Olivier Poirot <>
Submitted on : Wednesday, August 29, 2018 - 11:52:12 AM
Last modification on : Tuesday, January 14, 2020 - 1:48:30 PM
Contributor : Olivier Poirot <>
Submitted on : Wednesday, August 29, 2018 - 11:52:12 AM
Last modification on : Tuesday, January 14, 2020 - 1:48:30 PM
Identifiers
- HAL Id : hal-01863990, version 1
- DOI : 10.1111/1462-2920.14337
- PUBMED : 30063098
Collections
Citation
Kojadinovic-Sirinelli Mila, Villain Adrien, Puppo Carine, Fon Sing Sophie, Prioretti Laura, et al.. Exploring the microbiome of the “star” freshwater diatom Asterionella formosa in a laboratory context. Environmental Microbiology, Wiley-Blackwell, 2018, 20 (10), pp.3601-3615. ⟨10.1111/1462-2920.14337⟩. ⟨hal-01863990⟩
Metrics
Record views
