Fast magic-angle spinning NMR of membrane proteins
Résumé
Building on a decade of continuous advances of the community, the recent development of very fast (60 kHz and above) magic-angle spinning (MAS) probes has revolutionised the field of solid-state NMR. Today, rapid “fingerprinting” of proteins is possible with a ten-fold reduction of the required sample amounts with respect to conventional approaches, not only
in deuterated molecules but also in fully-protonated substrates. Extensive and robust resonance assignments can be derived rapidly for small-to-medium sized proteins (up to ca. 300 residues), opening the way to the determination of inter-nuclear proximities, relative orientations of secondary structural elements, protein-cofactor interactions, local and global
dynamics. Fast MAS and 1H detection techniques have nowadays been shown to be applicable to membrane-bound systems. This talk reviews the strategies underlying this recent leap forward in sensitivity and resolution, describing its potential for the detailed characterization of membrane proteins in lipid bilayers.