Addition of dairy lipids and probiotic Lactobacillus fermentum in infant formula programs gut microbiota and entero-insular axis in adult minipigs

Marion Lemaire (1, 2) , Samir Dou (3) , Armelle Cahu (1) , Michele Formal (1) , Laurence Le Normand (1) , Véronique Rome (1) , Isabelle Nogret (1) , Stéphanie Ferret-Bernard (1) , Moez Rhimi (4) , Isabelle Cuinet (2) , Cécile Canlet (5) , Marie Tremblay-Franco (5) , Pascale Le Ruyet (2) , Charlotte Baudry (2) , Philippe Gerard (4) , Sophie Blat (1) , Isabelle Le Huërou-Luron (1)

1 NuMeCan - Nutrition, Métabolismes et Cancer
2 Groupe Lactalis
3 PEGASE - Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes]
4 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
5 E20 - MetaToul AXIOM

Clinical and animal studies have demonstrated beneficial effects of early consumption of dairy lipids and a probiotic, Lactobacillus fermentum (Lf), on infant gut physiology. The objective of this study was to investigate their long-term effects on gut microbiota and host entero-insular axis and metabolism. Piglets were suckled with a milk formula containing only plant lipids (PL), a half-half mixture of plant lipids and dairy lipids (DL), or this mixture supplemented with Lf (DL + Lf). They were weaned on a standard diet and challenged with a high-energy diet until postnatal day 140. DL and DL + Lf modulated gut microbiota composition and metabolism, increasing abundance of several Clostridia genera. Moreover, DL + Lf specifically decreased the faecal content of 2-oxoglutarate and lysine compared to PL and 5-aminovalerate compared to PL and DL. It also increased short-chain fatty acid concentrations like propionate compared to DL. Furthermore, DL + Lf had a beneficial effect on the endocrine function, enhancing caecal GLP-1 and GLP-1 meal-stimulated secretion. Correlations highlighted the consistent relationship between microbiota and gut physiology. Together, our results evidence a beneficial programming effect of DL + Lf in infant formula composition on faecal microbiota and entero-insular axis function.

Mots clés: milk fat globules; nutritional imprinting; probiotic; milk fat globules

Mots clés: miniporc; immunité; microbiote digestif; probiotique; barrière épithéliale; nutrition; empreinte nutritionnelle; fonction endocrine; matière grasse du lait; miniporc

Domaines: Sciences du Vivant [q-bio]

Marion Lemaire, Samir Dou, Armelle Cahu, Michele Formal, Laurence Le Normand, et al.. Addition of dairy lipids and probiotic Lactobacillus fermentum in infant formula programs gut microbiota and entero-insular axis in adult minipigs. Scientific Reports, 2018, 8 (1), pp.11656. ⟨10.1038/s41598-018-29971-w⟩. ⟨hal-01858523⟩