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Article Dans Une Revue Journal of Chromatography A Année : 2017

The effect of high concentration additive on chiral separations in supercritical fluid chromatography

Résumé

Supercritical Fluid Chromatography is frequently used to efficiently handle separations of enantiomers. The separation of basic analytes usually requires the addition of a basic additive in the mobile phase to improve the peak shape or even to elute the compounds. The effect of increasing the concentration of 2-propylamine as additive on the elution of a series of basic compounds on a Chiralpak-AD stationary phase was studied. In this study, unusual additive concentrations ranging from 0.3% to 10% of 2-propylamine 2-propylaminein the modifier were explored and the effect on retention, peak shape, selectivity and resolution was evaluated. The addition of a large quantity of additive allowed to drastically improve the selectivity and the resolution, and even enantiomers elution order reversal was observed by changing the concentration of basic additive. The role of the ratio additive/modifier appeared a key to tune the enantioselectivity. Finally, the impact of these drastic conditions on the column material was evaluated.
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Dates et versions

hal-01857120 , version 1 (14-08-2018)

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David Speybrouck, Charline Doublet, Pascal Cardinaël, Catherine Fiol-Petit, David Corens. The effect of high concentration additive on chiral separations in supercritical fluid chromatography. Journal of Chromatography A, 2017, 1510, pp.89-99. ⟨10.1016/j.chroma.2017.06.049⟩. ⟨hal-01857120⟩
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