A Method for Radioactive Labelling of Hebeloma cylindrosporum to Study Plant-fungus Interactions

Adeline Becquer (1) , Margarita Torres Aquino (1) , Christine Le Guerneve (2) , Laurie Amenc (1) , Carlos Trives Segura (1) , Siobhan Staunton (1) , Herve Quiquampoix (1) , Claude Plassard (1)

1 UMR Eco&Sols - Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes
2 SPO - Sciences Pour l'Oenologie

In order to quantify P accumulation and P efflux in the ectomycorrhizal basidiomycete fungus Hebeloma cylindrosporum, we supplied 32P to mycelia previously grown in vitro in liquid medium. The culture had four main steps that are 1) growing the mycelium on complete medium with P, 2) transfer the mycelia into new culture solution with or without P, 3) adding a solution containing 32P and 4) rinsing the mycelia before incubation with or without plant. The main point is to rinse very carefully the mycelia after 32P supply in order to avoid overestimation of 32P efflux into the medium.

Mots clés: Phosphate availability; 32P labelling; Ectomycorrhizal fungi; in vitro culture

Mots clés: culture in vitro; hebeloma cylindrosporum; champignon ectomycorhizien; interaction plante champignon; biodisponibilité du phosphore

Domaines: Biologie végétale

Adeline Becquer, Margarita Torres Aquino, Christine Le Guerneve, Laurie Amenc, Carlos Trives Segura, et al.. A Method for Radioactive Labelling of Hebeloma cylindrosporum to Study Plant-fungus Interactions. Bio-protocol , 2017, 7 (20), ⟨10.21769/BioProtoc.2576⟩. ⟨hal-01837836⟩