ABCC1, an ATP Binding Cassette Protein from Grape Berry, Transports Anthocyanidin 3-O-Glucosides

Rita Maria Francisco (1, 2) , Ana Regalado (2) , Agnes Ageorges (3) , Bo J. Burla (1) , Barbara Bassin (1) , Cornelia Eisenach (1) , Olfa Zarrouk (2) , Sandrine Vialet (3) , Therese Marlin (3) , Maria Manuela Chaves (2) , Enrico Martinoia (1) , Reka Nagya (1)

1 Inst Plant Biol
2 ITQB - Instituto de Tecnologia Química e Biológica António Xavier
3 SPO - Sciences Pour l'Oenologie

Accumulation of anthocyanins in the exocarp of red grapevine (Vitis vinifera) cultivars is one of several events that characterize the onset of grape berry ripening (veraison). Despite our thorough understanding of anthocyanin biosynthesis and regulation, little is known about the molecular aspects of their transport. The participation of ATP binding cassette (ABC) proteins in vacuolar anthocyanin transport has long been a matter of debate. Here, we present biochemical evidence that an ABC protein, ABCC1, localizes to the tonoplast and is involved in the transport of glucosylated anthocyanidins. ABCC1 is expressed in the exocarp throughout berry development and ripening, with a significant increase at veraison (i.e., the onset of ripening). Transport experiments using microsomes isolated from ABCC1-expressing yeast cells showed that ABCC1 transports malvidin 3-O-glucoside. The transport strictly depends on the presence of GSH, which is cotransported with the anthocyanins and is sensitive to inhibitors of ABC proteins. By exposing anthocyanin-producing grapevine root cultures to buthionine sulphoximine, which reduced GSH levels, a decrease in anthocyanin concentration is observed. In conclusion, we provide evidence that ABCC1 acts as an anthocyanin transporter that depends on GSH without the formation of an anthocyanin-GSH conjugate.

Mots clés: glutathione s transferases; time rt pcr; flavonobiosynthesis; arabidopsis thaliana; vacuolar inclusions; medicago truncatula; maximum likelihood; gene expression; seed coat; sequestration

Domaines: Sciences du Vivant [q-bio]

Rita Maria Francisco, Ana Regalado, Agnes Ageorges, Bo J. Burla, Barbara Bassin, et al.. ABCC1, an ATP Binding Cassette Protein from Grape Berry, Transports Anthocyanidin 3-O-Glucosides. The Plant cell, 2013, 25 (5), pp.1840 - 1854. ⟨10.1105/tpc.112.102152⟩. ⟨hal-01837678⟩