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Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa

Pauline Bernardo 1, 2 Brejnev Muhire 3 Sarah François 4, 1, 5, 6, 7, 8, 9 Maelle Deshoux 1 Penelope Hartnady 3 Kata Farkas 10 Simona Kraberger 10 Denis Filloux 1, 2 Emmanuel Fernandez 1, 2 Serge Galzi 1, 2 Romain Ferdinand 1, 2 Martine Granier 1, 2 Armelle Marais 11, 12 Pablo Monge Blasco 13 Thierry Candresse 11, 12 Fernando Escriu 14 Arvind Varsani 10, 15, 16 Gordon W. Harkins 17 Darren P. Martin 3 Philippe Roumagnac 1, 2
Abstract : Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae, severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region.
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https://hal.archives-ouvertes.fr/hal-01837296
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Submitted on : Thursday, July 12, 2018 - 8:04:31 PM
Last modification on : Friday, September 4, 2020 - 3:06:25 PM

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Pauline Bernardo, Brejnev Muhire, Sarah François, Maelle Deshoux, Penelope Hartnady, et al.. Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virology, Elsevier, 2016, 493, pp.142-153. ⟨10.1016/j.virol.2016.03.016⟩. ⟨hal-01837296⟩

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