MitoCeption as a new tool to assess the effects of mesenchymal stem/stromal cell mitochondria on cancer cell metabolism and function
Résumé
Mitochondrial activity is central to tissue homeostasis. Mitochondria dysfunction constitutes a hallmark of
many genetic diseases and plays a key role in tumor progression. The essential role of mitochondria, added
to their recently documented capacity to transfer from cell to cell, obviously contributes to their current
interest. However, determining the proper role of mitochondria in defined biological contexts was
hampered by the lack of suitable experimental tools. We designed a protocol (MitoCeption) to directly and
quantitatively transfer mitochondria, isolated from cell type A, to recipient cell type B. We validated and
quantified the effective mitochondria transfer by imaging, fluorescence-activated cell sorting (FACS) and
mitochondrial DNA analysis. We show that the transfer of minute amounts of mesenchymal stem/stromal
cell (MSC) mitochondria to cancer cells, a process otherwise occurring naturally in coculture, results in
cancer cell enhanced oxidative phosphorylation (OXPHOS) activity and favors cancer cell proliferation and
invasion. The MitoCeption technique, which can be applied to different cell systems, will therefore be a
method of choice to analyze the metabolic modifications induced by exogenous mitochondria in host cells.
Domaines
Sciences du Vivant [q-bio]
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