Xurography-based microfluidic algal biosensor and dedicated portable measurement station for online monitoring of urban polluted samples

Abstract : A critical need exists to develop rapid, in situ, and real-time tools to monitor the impact of pollution discharge toxicity on aquatic ecosystems. The present paper deals with the development of a novel, simple-to-use, low-cost, portable, and user-friendly algal biosensor. In this study, a complete and autonomous portable fluorimeter was developed to assess the A-chlorophyll fluorescence of microalgae, inserted by capillarity into low-cost and disposable xurography-based microfluidic chips. Three microalgae populations were used to develop the biosensor: Chlorella vulgaris, Pseudokirchneriella subcapitata, and Chlamydomonas reinhardtii. Biosensor feasibility and sensitivity parameters, such as algal concentration and light intensity, were optimized beforehand to calibrate the biosensor sensitivity with Diuron, a pesticide known to be very toxic for microalgae. Finally, the biosensor was employed in 10 aqueous urban polluted samples (7 urban wet-weather discharges and 3 wastewaters) in order to prove its reliability, reproducibility, and performance in the detection of toxic discharges in the field.
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Submitted on : Friday, July 6, 2018 - 8:04:52 PM
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Antoine Gosset, Claude Durrieu, Louis Renaud, Anne-Laure Deman, Pauline Barbe, et al.. Xurography-based microfluidic algal biosensor and dedicated portable measurement station for online monitoring of urban polluted samples. Biosensors and Bioelectronics, Elsevier, 2018, 117, pp.669-677. ⟨10.1016/j.bios.2018.07.005⟩. ⟨hal-01832289⟩

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