Rapid detection of Staphylococcus aureus and methicillin resistance in bone and joint infection samples: evaluation of the GeneXpert MRSA/SA SSTI assay

Florent Valour (1) , Viviane Blanc-Pattin (2) , Anne-Marie Freydiere (3) , Anissa Bouaziz (4) , Emmanuel Chanard (5) , Sebastien Lustig (6) , Tristan Ferry (4) , Frédéric Laurent (7)

1 INSERM U1111
2 Laboratory of bacteriology, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France
3 Laboratory of bacteriology, East Lyon University Hospital, Hospices Civils de Lyon, Lyon, France
4 Department of infectious diseases, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France
5 Novescia
6 LBMC UMR T9406 - Laboratoire de Biomécanique et Mécanique des Chocs
7 French National Reference Center for Staphylococci, Hospices Civils de Lyon, Lyon, France

The GeneXpert MRSA/SA SSTI assay was compared to conventional cultures to detect Staphylococcus aureus and methicillin-resistance from 91 bone and joint infection samples. Sensitivity and specificity were 94.4% and 100%. Three false-positive results were observed, in fact providing from patients known to be infected by S. aureus on the basis of other concomitant osteoarticular samples, which suggests that PCR was more sensitive than culture. This diagnosis accuracy may help shorten toxic and non-optimal empirical therapies such as glycopeptides in case of methicillin-susceptible strains.

Mots clés: STAPHYLOCOCCUS AUREUS; BONE AND JOINT INFECTION; METHICILLIN-RESISTANCE; REAL TIME POLYMERASE CHAIN REACTION; GENEXPERT MRSA/SA SSTI ASSAY

Mots clés: BIOMECANIQUE; OS

Domaines: Biomécanique [physics.med-ph]

Florent Valour, Viviane Blanc-Pattin, Anne-Marie Freydiere, Anissa Bouaziz, Emmanuel Chanard, et al.. Rapid detection of Staphylococcus aureus and methicillin resistance in bone and joint infection samples: evaluation of the GeneXpert MRSA/SA SSTI assay. Diagnostic Microbiology and Infectious Disease, 2014, 78 (3), pp. 313-315. ⟨10.1016/j.diagmicrobio.2013.11.026⟩. ⟨hal-01823803⟩