New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells

Javier Jiménez-Lamana (1) , Joanna Szpunar (1) , Ryszard Lobinski (1)

1 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Single cells represent the basic building units of life, and thus their study is one the most important areas of research. However, classical analysis of biological cells eludes the investigation of cell-to-cell differences to obtain information about the intracellular distribution since it only provides information by averaging over a huge number of cells. For this reason, chemical analysis of single cells is an expanding area of research nowadays. In this context, metallomics research is going down to the single-cell level, where high-resolution high-sensitive analytical techniques are required. In this chapter, we present the latest developments and applications in the fields of single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS), mass cytometry, laser ablation (LA)-ICP-MS, nanoscale secondary ion mass spectrometry (nanoSIMS), and synchrotron X-ray fluorescence microscopy (SXRF) for single-cell analysis. Moreover, the capabilities and limitations of the current analytical techniques to unravel single-cell metabolomics as well as future perspectives in this field will be discussed.

Mots clés: Single-cell analysis; ICP-MS; Laser ablation; NanoSIMS; X-ray fluorescence; Imaging; Intracellular distribution; Metal content

Domaines: Sciences du Vivant [q-bio]

Javier Jiménez-Lamana, Joanna Szpunar, Ryszard Lobinski. New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells. Arruda M. Metallomics:The Science of Biometals, 1055, Springer International , pp.245 - 270, 2018, ⟨10.1007/978-3-319-90143-5_10⟩. ⟨hal-03133546⟩