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, All samples displayed positivity for the brush border markers. Scale = 25 µm. These experiments were repeated three times independently and findings were similar. (e) Normalized FPKMs were plotted for tHIO, tHIO+S and adult jejunum for SI and DPP4. Significant increases in transcripts were found in tHIO+S when compared to tHIO. (f) Corrected short circuit current of tHIO, tHIO+S and adult jejunum was plotted. A decreasing trend is observed, but changes are not statistically significant.. (g) Corrected calculated FITC dextran flux for tHIO, tHIO+S and adult jejunum was plotted. Flux was significantly decreased in tHIO+S compared to tHIO and trended toward the level of adult jejunum. (h) Corrected transepithelial resistance of tHIO, tHIO+S and adult jejunum was plotted. Observations across groups were similar. For (e-h) sample sizes are the following: tHIO n=5, tHIO+S n=4, and adult jejunum n=2. All samples are biologically independent. Data are represented as the mean ± SD. An ANOVA followed by Tukey's post hoc tests were performed and the statistical significance cutoff was p < 0.05. (i) Normalized FPKMs were plotted for tHIO, tHIO+S and adult jejunum for tight junction components Tight Junction Protein 1 (TJP1), Junctional Adhesion Molecule 1 (F11R) and Metadherin (MTDH). For F11R and MTDH, the expression level in tHIO+S was significantly increased above that in the tHIO, n=4 and adult jejunum n=3. All samples are biologically independent. (d) Scaled Centered Principal Component Analysis of samples retrieved from our study and several publicly available databases. Sample sizes are the following: fetal small intestine n=5, infant small intestine n=6, child small intestine n=1, adult small intestine n=9, HIO-H1 n=5, HIO-H9 n=3, tHIO n=6, and tHIO+S n=4