Microencapsulation of rifampicin for the prevention of endophthalmitis: In vitro release studies and antibacterial assessment

Abstract : Rifampicin encapsulated microparticles were designed for intraocular injection after cataract surgery to prevent postoperative endophthalmitis. Microparticles were formulated by emulsification diffusion method using poly(lactic acid-co-glycolic acid) (PLGA) as polymer in order to propose a new form of rifampicin that overcome its limitations in intraocular delivery. Depending on processing formulation, different types of microparticles were prepared, characterized and evaluated by in vitro release studies. Two types of microparticles were selected to get a burst release of rifampicin, to reach minimal inhibitory concentrations to inhibit 90% of Staphylococcus epidermidis mainly involved in postoperative endophthalmitis, combined with a sustained release to maintain rifampicin concentration over 24 h. The antibacterial activity and antiadhesive property on intraocular lenses were evaluated on S. epidermidis. Microparticles, with a rapid rifampicin release profile, showed an effect towards bacteria development similar to free rifampicin over 48 h. However, slow-release profile microparticles exhibited a similar antibacterial effect during the first 24 h, and were able to destroy all the S epidermidis in the medium after 30 h. The association of the two formulations allowed obtaining interesting antibacterial profile. Moreover, rifampicin-loaded microparticles have shown a very efficient anti-adherent effect of S. epidermidis on intraocular lenses at 24 h. These results propose rifampicin microparticles as suitable for antibioprophylaxis of the postoperative endophthalmitis. (C) 2016 Elsevier B.V. All rights reserved.
Type de document :
Article dans une revue
International Journal of Pharmaceutics, Elsevier, 2016, 505 (1-2), pp.262-270. 〈10.1016/j.ijpharm.2016.03.026〉
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01814080
Contributeur : Laboratoire Mateis <>
Soumis le : mardi 12 juin 2018 - 17:57:39
Dernière modification le : mardi 11 décembre 2018 - 11:22:05

Identifiants

Collections

Citation

Mi Yeon Lee, Sandrine Bourgeois, Eyad Almouazen, Jocelyne Pelletier, Francois Renaud, et al.. Microencapsulation of rifampicin for the prevention of endophthalmitis: In vitro release studies and antibacterial assessment. International Journal of Pharmaceutics, Elsevier, 2016, 505 (1-2), pp.262-270. 〈10.1016/j.ijpharm.2016.03.026〉. 〈hal-01814080〉

Partager

Métriques

Consultations de la notice

62