M. J. Briones, P. Ineson, and T. V. Piearce, Effects of climate change on soil fauna, 1997.

, Diptera larvae and tardigrades in a transplant experiment, Applied Soil Ecology, vol.6, issue.2, pp.117-134

M. J. Briones, P. Ineson, and A. Heinemeyer, Predicting potential impacts of climate change on the geographical distribution of enchytraeids: a meta-analysis approach, Global Change Biology, vol.13, issue.11, pp.2252-2269, 2007.

M. J. Briones, M. H. Garnett, and P. Ineson, Soil biology and warming play a key role in the release of "old C" from organic soils, Soil Biology and Biochemistry, vol.42, issue.6, pp.4-6, 2010.

W. Didden and J. Römbke, Enchytraeids as Indicator Organisms for Chemical Stress in Terrestrial Ecosystems, Ecotoxicology and Environmental Safety, vol.50, issue.1, pp.25-43, 2001.

S. Gomes, S. Novais, C. Gravato, L. Guilhermino, J. Scott-fordsmand et al., Effect of Cu-nanoparticles versus one Cu-salt: Analysis of stress biomarkers response in Enchytraeus albidus (Oligochaeta), Nanotoxicology, vol.6, issue.2, pp.134-143, 2011.

, ISO (International Organisation for Standardization) 2014. Soil quality -Effects of contaminants on Enchytraeidae-(Enchytraeus sp.) determination of effects on reproduction

R. Kuperman, M. Amorim, J. Römbke, R. Lanno, R. Checkai et al., Adaptation of the enchytraeid toxicity test for use with natural soil types, European Journal of Soil Biology, vol.42, pp.234-243, 2006.

J. Römbke and T. Moser, Validating the enchytraeid reproduction test: organisation and results of an international ringtest, Chemosphere, vol.46, issue.7, pp.1117-1140, 2002.

L. Zhang and C. Van-gestel, The toxicity of different lead salts to Enchytraeus crypticus in relation to bioavailability in soil, The study was financed by The National Science Centre, vol.36, pp.2083-2091, 2017.

A. Bieganowski, , 2018.

C. J. Bronick and R. Lal, Geoderma, vol.124, pp.3-22, 2005.

W. D. Kemper and R. C. Rosenau, W Klute (red.) Methods of soil analysis Part 1, 1986.

. Pn-iso, , 2001.

S. D. Saygin and . In, Land Degrad. Develop, vol.28, pp.199-206, 2017.

J. Six, Soil Biol. Biochem, vol.32, pp.2099-2103, 2000.

R. S. Swift, Methods of soil analysis, 1996.

A. Zsolany, Geoderma, vol.113, pp.187-209, 2003.

K. B. Gongalsky, Stratification and dynamics of bait-lamina perforation in three forest soils along a north-south gradient in Russia, Key words: microcosm, aggregate stability, straw, humid acid, compost, peat, vol.25, pp.111-122, 2004.

K. B. Gongalsky, T. Persson, and A. D. Pokarzhevskii, Effects of soil temperature and moisture on the feeding activity of soil animals as determined by the bait-lamina test, Applied Soil Ecology, vol.39, issue.1, pp.84-90, 2008.

B. Helling, G. Pfeiff, and O. Larink, A comparison of feeding activity of collembolan and enchytraeid in laboratory studies using the bait-lamina test, Applied Soil Ecology, vol.7, pp.207-212, 1998.

, Soil quality -Method for testing effects of soil contaminant on the feeding activity of soil dwelling organisms -Bait-lamina test, International Organization for Standardization, 2015.

W. Kratz, The bait-lamina test: General aspects, applications and perspectives. Environmental science and pollution research international, vol.5, pp.94-96, 1998.

O. Larink, Bait lamina as a tool for testing the feeding activity of animals in contaminated soils, Ecotoxicology of Soil Organisms, pp.339-345, 1993.

J. E. Simpson, Factors affecting soil fauna feeding activity in a fragmented lowland temperate deciduous woodland, PLoS ONE, vol.7, issue.1, 2012.

C. A. Van-gestel, M. Kruidenier, and M. P. Berg, Suitability of wheat straw decomposition, cotton strip degradation and bait-lamina feeding tests to determine soil invertebrate activity, Biology and Fertility of Soils, vol.37, pp.115-123, 2003.

C. A. Van-gestel, The use of acute and chronic bioassays to determine the ecological risk and bioremediation efficiency of oil-polluted soils, Environmental Toxicology and Chemistry, vol.20, issue.7, pp.1438-1449, 2001.

E. Von-törne, Assessing feeding activities of soil-living animals I. Bait-lamina-tests, 1990.

, Pedobiologia, vol.34, pp.89-101

, The other species, representing less than 5 % of the total abundance, were: Achaeta bohemica (Vejdovský, 1879)

A. Eiseni,

, Achaeta pannonica (Graefe, 1989.

, Buchholzia fallax

, Enchytraeus buchholzi s.l. (Vejdovský, 1879)

E. Parva, , 1959.

C. Nielsen, Fridericia bulboides, 1959.

F. Isseli, , 1994.

F. Perrieri, Vejdovský, 1878) and Marionina communis, 1959.

R. M. Schmelz and R. Collado, A guide to European terrestrial and freshwater species of Enchytraeidae (Oligochaeta), Soil Org, vol.82, pp.1-176, 2010.

, Key words: Long-term experiment, fertilization, earthworms, enchytraeids

R. C. Bicho, F. C. Santos, J. J. Scott-fordsmand, and M. J. Amorim, Multigenerational effects of copper nanomaterials (CuONMs) are different of those of CuCl 2 : exposure in the soil invertebrate Enchytraeus crypticus, 2017.

R. C. Bicho, F. C. Santos, M. F. Goncalves, A. M. Soares, and M. J. Amorim, , 2015.

, Enchytraeid Reproduction Test(PLUS): hatching, growth and full life cycle test-an optional multi-endpoint test with Enchytraeus crypticus, Ecotoxicology, vol.24, pp.1053-1063

S. I. Gomes, C. P. Roca, N. Pegoraro, T. Trindade, J. J. Scott-fordsmand et al., Nanotoxicology

S. I. Gomes, C. P. Roca, J. J. Scott-fordsmand, and M. J. Amorim, High-throughput transcriptomics reveals uniquely affected pathways: AgNPs, PVP-coated AgNPs and Ag NM300K case studies, Environmental Science-nano, vol.4, pp.929-937, 2017.

S. I. Gomes, A. M. Soares, and M. J. Amorim, Effect of Cu and Ni on cellular energy allocation in Enchytraeus albidus, Ecotoxicology, vol.25, pp.1523-1530, 2016.

M. F. Gonçalves, R. C. Bicho, A. Rema, A. M. Soares, A. M. Faustino et al., Development of an embryotoxicity test for Enchytraeus crypticus -The effect of Cd, Chemosphere, vol.139, pp.386-392, 2015.

C. F. Howcroft, M. J. Amorim, C. Gravato, L. Guilhermino, and A. M. Soares, Effects of natural and chemical stressors on Enchytraeus albidus: Can oxidative stress parameters be used as fast screening tools for the assessment of different stress impacts in soils? Environment International, vol.35, pp.318-324, 2009.

V. L. Maria, M. J. Ribeiro, S. Guilherme, A. M. Soares, and J. J. Scott-fordsmand,

M. J. Amorim, Silver (nano)materials cause genotoxicity in Enchytraeus crypticus -as determined by the comet assay, Environmental Toxicology And Chemistry, vol.37, pp.184-191, 2018.

L. A. Mendes, M. J. Amorim, and J. J. Scott-fordsmand, Interactions of Soil Species Exposed to CuO NMs are Different From Cu Salt -A Multispecies Test, Environmental Science And Technology, vol.52, pp.4413-4421, 2018.

, Key words : Omics, Population, Full life cycle