Histone methylation changes are required for life cycle progression in the human parasite Schistosoma mansoni - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS Pathogens Année : 2018

Histone methylation changes are required for life cycle progression in the human parasite Schistosoma mansoni

Résumé

Epigenetic mechanisms and chromatin structure play an important role in development. Their impact is therefore expected to be strong in parasites with complex life cycles and multiple , strikingly different, developmental stages, i.e. developmental plasticity. Some studies have already described how the chromatin structure, through histone modifications, varies from a developmental stage to another in a few unicellular parasites. While H3K4me3 profiles remain relatively constant, H3K27 trimethylation and bivalent methylation show strong variation. Inhibitors (A366 and GSK343) of H3K27 histone methyltransferase activity in S. mansoni efficiently blocked miracidium to sporocyst transition indicating that H3K27 tri-methylation is required for life cycle progression. As S. mansoni is a multicellular parasite that significantly affects both the health and economy of endemic areas, a better understanding of fluke developmental processes within the definitive host will likely highlight novel disease control strategies. Towards this goal, we also studied H4K20me1 in female cercar-iae and adults. In particular, we found that bivalent trimethylation of H3K4 and H3K27 at the transcription start site of genes is a landmark of the cercarial stage. In cercariae, H3K27me3 presence and strong enrichment in H4K20me1 over long regions (10–100 kb) is associated with development related genes. Here, we provide a broad overview of the chromatin structure of a metazoan parasite throughout its most important lifecycle stages. The five developmental stages studied here present distinct chromatin structures, indicating that histone methylation plays an important role during development. Hence, components of the histone methylation (and demethylation) machinery may provide suitable Schistosomiasis control targets

Domaines

Parasitologie
Fichier principal
Vignette du fichier
Roquis-2018-PlosPatho-Histone.pdf (8.43 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01808531 , version 1 (05-06-2018)

Identifiants

Citer

David Roquis, Aaron Taudt, Kathrin P Geyer, Gilda Padalino, Karl F Hoffmann, et al.. Histone methylation changes are required for life cycle progression in the human parasite Schistosoma mansoni. PLoS Pathogens, 2018, 14 (5), pp.e1007066. ⟨10.1371/journal.ppat.1007066⟩. ⟨hal-01808531⟩
146 Consultations
88 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More