UPLC-MS/MS quantitative analysis and structural fragmentation study of five Parmotrema lichens from the Eastern Ghats

K Kumar (1) , Bandi Siva (1) , V U M Sarma (1) , Satish Mohabe , a Madhusudana Reddy , Joël Boustie (2) , Ashok K Tiwari (1) , N Rama Rao , K Suresh Babu (1)

1 IICT - CSIR Indian Institute of Chemical Technology
2 ISCR - Institut des Sciences Chimiques de Rennes

Comparative phytochemical analysis of five lichen species [Parmotrema tinctorum (Delise ex Nyl.) Hale, P. andinum (Mull. Arg.) Hale, P. praesorediosum (Nyl.) Hale, P. grayanum (Hue) Hale, P. austrosinense (Zahlbr.) Hale] of Parmotrema genus were performed using two complementary UPLC-MS systems. The first system consists of high resolution UPLC-QToF-MS/MS spectrometer and the second system consisted of UPLC-MS/MS in Multiple Reaction Monitoring (MRM) mode for quantitative analysis of major constituents in the selected lichen species. The individual compounds (47 compounds) were identified using Q-ToF-MS/MS, via comparison of the exact molecular masses from their MS/MS spectra, the comparison of literature data and retention times to those of standard compounds which were isolated from crude extract of abundant lichen, P. tinctorum. The analysis also allowed us to identify unknown peaks/compounds, which were further characterized by their mass fragmentation studies. The quantitative MRM analysis was useful to have a better discrimination of species according to their chemical profile. Moreover, the determination of antioxidant activities (ABTS inhibition) and Advance Glycation Endproducts (AGEs) inhibition carried out for the crude extracts revealed a potential antiglycaemic activity to be confirmed for P. austrosinense.

Mots clés: Parmotrema austrosinense; Parmotrema andinum; Parmeliaceae; Metabolite profiling; Parmotrema praesorediosum; Parmotrema tinctorum; Parmotrema grayanum; Fragmentation studies; Matrix solid-phase dispersion; UPLC-ToF-MS/MS

Domaines: Chimie thérapeutique; Biochimie [q-bio.BM]

K Kumar, Bandi Siva, V U M Sarma, Satish Mohabe, a Madhusudana Reddy, et al.. UPLC-MS/MS quantitative analysis and structural fragmentation study of five Parmotrema lichens from the Eastern Ghats. Journal of Pharmaceutical and Biomedical Analysis, 2018, 156, pp.45-57. ⟨10.1016/j.jpba.2018.04.017⟩. ⟨hal-01807884⟩