Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry.

Elisabetta Boeri Erba (1) , Luca Signor (1) , Mizar Francesca Oliva (1) , Fabienne Hans (1) , Carlo Petosa (1)

1 IBS - UMR 5075 - Institut de biologie structurale

Native mass spectrometry (MS) enables the characterization of macromolecular assemblies with high sensitivity. It can reveal the stoichiometry of subunits as well as their two-dimensional interaction network and provide information regarding the dynamic behavior of macromolecular complexes. Here, we describe the workflow to perform native MS experiments. In addition, we illustrate the quality control analysis of proteins using MS in denaturing conditions.

Mots clés: Macromolecular assemblies; Native mass spectrometry; Stoichiometry; Two-dimensional map of interactions

Domaines: Biologie structurale [q-bio.BM]

Elisabetta Boeri Erba, Luca Signor, Mizar Francesca Oliva, Fabienne Hans, Carlo Petosa. Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry.. Methods in Molecular Biology, 2018, 1764, pp.133-151. ⟨10.1007/978-1-4939-7759-8_9⟩. ⟨hal-01807803⟩