HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Toscana virus nucleoprotein oligomer organization observed in solution

Abstract : Toscana virus (TOSV) is an arthropod-borne virus belonging to the Phlebovirus genus within the Bunyaviridae family. As in other bunyaviruses, the genome of TOSV is made up of three RNA segments. They are encapsidated by the nucleoprotein (N), which also plays an essential role in virus replication. To date, crystallographic structures of phlebovirus N have systematically revealed closed-ring organizations which do not fully match the filamentous organization of the ribonucleoprotein (RNP) complex observed by electron microscopy. In order to further bridge the gap between crystallographic data on N and observations of the RNP by electron microscopy, the structural organization of recombinant TOSV N was investigated by an integrative approach combining X-ray diffraction crystallography, transmission electron microscopy, small-angle X-ray scattering, size-exclusion chromatography and multi-angle laser light scattering. It was found that in solution TOSV N forms open oligomers consistent with the encapsidation mechanism of phlebovirus RNA.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-01802854
Contributor : Yves Bourne Connect in order to contact the contributor
Submitted on : Tuesday, May 29, 2018 - 6:52:18 PM
Last modification on : Monday, January 31, 2022 - 5:12:07 PM

Identifiers

Collections

Citation

Amal Baklouti, Adeline Goulet, Julie Lichiere, Bruno Canard, Remi Charrel, et al.. Toscana virus nucleoprotein oligomer organization observed in solution. Acta crystallographica Section D : Structural biology [1993-..], International Union of Crystallography, 2017, 73 (8), pp.650 - 659. ⟨10.1107/S2059798317008774⟩. ⟨hal-01802854⟩

Share

Metrics

Record views

49