Molecular investigation of iron-sulfur cluster assembly scaffolds under stress

Béatrice Blanc (1) , Martin Clémancey (1) , Jean-Marc Latour (1) , Marc Fontecave (1, 2) , Sandrine Ollagnier de Choudens (1)

1 LCBM - UMR 5249 - Laboratoire de Chimie et Biologie des Métaux
2 Collège de France - Chaire Chimie des processus biologiques

Abstract Fe/S biosynthesis is controlled in Escherichia coli by two machineries, the housekeeping ISC machinery and the SUF system that is functional under stress conditions. Despite many in vivo studies showing that SUF is more adapted for Fe/S assembly under stress, no molecular data supporting this concept have been provided so far. This work focuses on molecular studies of key actors in Fe/S assembly, the SufB and IscU scaffolds under oxidative stress and iron limitation. We show that the IscU Fe2S2 cluster is less stable than the SufB Fe2S2 cluster in the presence of hydrogen peroxide, oxygen, and an iron chelator.

Domaines: Biochimie, Biologie Moléculaire

Béatrice Blanc, Martin Clémancey, Jean-Marc Latour, Marc Fontecave, Sandrine Ollagnier de Choudens. Molecular investigation of iron-sulfur cluster assembly scaffolds under stress. Biochemistry, 2014, 53 (50), pp.7867-7869. ⟨10.1021/bi5012496⟩. ⟨hal-01801678⟩