High Expression of Fas/CD95 on CD4+ Circulating T Cells: An Exclusion Criterion in the Diagnosis of Mycosis Fungoides?

Aurélie Du-Thanh (1, 2) , Pierre Portales (1) , Anne Serre-Cousiné (1, 2) , Céline Girard (1, 2) , Bernard Guillot (1, 2) , Olivier Dereure (1, 2)

1 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
2 Pathogénèse et contrôle des infections chroniques (PCCI)

The aim of this 10-year monocentric prospective study was to determine a cut-off value of Fas/CD95 expression by peripheral blood CD4+ T lymphocytes in discriminating patients with mycosis fungoides from controls with cutaneous benign lymphocytic conditions. CD95 expression in peripheral blood CD4+ T lymphocytes was measured using flow cytometry in 330 patients referred for diagnosis: 104 with mycosis fungoides and 226 with eczema, psoriasis, drug reaction, etc. The sensitivity and specificity of different thresholds of CD95 expression were calculated regarding the final diagnosis of patients with mycosis fungoides or controls. CD95 expression higher than 30% reached a specificity of 91% in ruling out a diagnosis of mycosis fungoides, although overall CD95 expression was not significantly different from that of controls (p = 0.309) and sensitivity was very low (5%). Thus, peripheral CD95 expression higher than 30% could be used among the exclusion criteria in a multicomponent score for mycosis fungoides diagnosis.

Mots clés: peri pheral blood CD4 + CD95 + rate; CD95 expression; Fas/Fas ligand system; mycosis fungoides

Domaines: Dermatologie; Maladies infectieuses; Immunité adaptative

Aurélie Du-Thanh, Pierre Portales, Anne Serre-Cousiné, Céline Girard, Bernard Guillot, et al.. High Expression of Fas/CD95 on CD4+ Circulating T Cells: An Exclusion Criterion in the Diagnosis of Mycosis Fungoides?. Acta Dermato-Venereologica, 2017, 97 (7), pp.834 - 837. ⟨10.2340/00015555-2632⟩. ⟨hal-01783445⟩