Microbial decomposition of large organic particles in the northwestern Mediterranean Sea:an experimental approach

Richard Sempere (1, 2, 3) , Sc Yoro (1) , France van Wambeke (1, 2) , Bruno Charrìère (1)

1 LMGEM - Laboratoire de MicrobiologiE de Géochimie et d'Ecologie Marines
2 MIO - Institut méditerranéen d'océanologie
3 AMU SCI - Aix-Marseille Université - Faculté des Sciences

Sediment trap particles, as well as particulate organic material including particles larger than 10 pn coiiected by in situ pumps (ISP) and fresh corpses of the gelatinous zoopIankton species Thalia democratica, were coiiected in the northwestern Mediterranean Sea from Apnl to July 1995, and incubated (after mixing with 0.2 pn filtered seawater) under laboratory conditions with their own bac-tenal assemblages for 6 to 24 d in batches under oxic conditions and in the dark. Particulate (POC > 0.7 pm), dissolved (DOC < 0.7 p n) and colloidal(0.02 < COC < 0.7 pm) organic carbon contents, as weil as bactenal abundance and production, were quantified over time. In all experiments, total organic carbon (TOC = POC + DOC) decrease covaned with an increase in bactenal abundance and production, bactena being the main mediators of particle decomposition. We found that COC accounted for 19 to 31 % of DOC immediately after particle dilution in 0.2 pm filtered seawater, and always for less than 9 % at the end of the experiments. As organic colloids compnsed less than 7 % of DOC in the 0.2 pn filtered seawater used to dilute the particles, this result suggests that COC was mainly produced from particle decomposition. Assuming that bactenal populations were the sole decomposer of organic matter in the batches, the results gave bacterial growth efficiencies (BGE) in the range of 3 to 21%, indicating that decomposition of these particles significantly produce CO2 through bacterial respiration. The results showed that bactena degraded 16 to 87 % of the initial amount of POC within the first 48 h, whereas only 6 to 22 % of POC was degraded in the second stages. Our data and modeling work based on such short incubation times suggest that salp bodies are composed of 1 labile and 1 refractory organic fraction, whereas both 1SP-and trap particles are composed of 2 labile and 1 refractory organic fraction. A lG-model (for salp) and a 2G-model for other particles was able to satisfactorily reproduce the data sets.

Mots clés: carbon; sinking particles; bacterial decomposition; Marine organic particles

Domaines: Sciences de l'environnement; Océan, Atmosphère; Sciences du Vivant [q-bio]

Richard Sempere, Sc Yoro, France van Wambeke, Bruno Charrìère. Microbial decomposition of large organic particles in the northwestern Mediterranean Sea:an experimental approach. Marine Ecology Progress Series, 2000, 198, pp.61 - 72. ⟨10.3354/meps198061⟩. ⟨hal-01780471⟩