Ultraviolet, Infrared, and High-Low Energy Photodissociation of Post-Translationally Modified Peptides

Abstract : Mass spectrometry-based methods have made significant progress in characterizing post-translational modifications in peptides and proteins; however, certain aspects regarding fragmentation methods must still be improved. A good technique is expected to provide excellent sequence information, locate PTM sites, and retain the labile PTM groups. To address these issues, we investigate 10.6 μm IRMPD, 213 nm UVPD, and combined UV and IR photodissociation, known as HiLoPD (high-low photodissociation), for phospho-, sulfo-, and glyco-peptide cations. IRMPD shows excellent backbone fragmentation and produces equal numbers of N- and C-terminal ions. The results reveal that 213 nm UVPD and HiLoPD methods can provide diverse backbone fragmentation producing a/x, b/y, and c/z ions with excellent sequence coverage, locate PTM sites, and offer reasonable retention efficiency for phospho- and glyco-peptides. Excellent sequence coverage is achieved for sulfo-peptides and the position of the SO3 group can be pinpointed; however, widespread SO3 losses are detected irrespective of the methods used herein. Based on the overall performance achieved, we believe that 213 nm UVPD and HiLoPD can serve as alternative options to collision activation and electron transfer dissociations for phospho- and glyco-proteomics. Graphical Abstract ᅟ.
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Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2018, 29 (2), pp.270 - 283. 〈10.1007/s13361-017-1794-9〉
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Soumis le : lundi 16 avril 2018 - 14:11:45
Dernière modification le : mardi 17 avril 2018 - 01:22:24

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Mohammad Halim, Luke Macaleese, Jérôme Lemoine, Rodolphe Antoine, Philippe Dugourd, et al.. Ultraviolet, Infrared, and High-Low Energy Photodissociation of Post-Translationally Modified Peptides. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2018, 29 (2), pp.270 - 283. 〈10.1007/s13361-017-1794-9〉. 〈hal-01767664〉

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