Physico-chemical characterization of the interaction of red fluorescent protein — DsRed with silica layers

Marvine Soumbo (1, 2) , Alessandro Pugliara (1, 3) , Marie-Carmen Monje (2) , Christine Roques (2) , Bernard Despax (1) , Caroline Bonafos (3) , Robert Carles (3) , Adnen Mlayah (3) , Kremena Makasheva (4)

1 LAPLACE - LAboratoire PLasma et Conversion d'Energie
2 LGC - Laboratoire de Génie Chimique
3 CEMES - Centre d'élaboration de matériaux et d'études structurales
4 LAPLACE-MPP - Matériaux et Procédés Plasmas

The Discosoma Recombinant Red Fluorescent (DsRed) protein is the latest member of the family of fluorescent proteins. It holds great promise for applications in biotechnology and cell biology. However, before being used for rational engineering, knowledge on the underlying mechanisms relating the DsRed structural stability and adsorption properties on solid surfaces is highly demanded. The physico-chemical analysis performed in this study reveals that the interaction of DsRed with SiO2 surfaces does not lead to protein denaturation. The secondary structure of DsRed is preserved after adsorption and dehydration. The measured contact angles of sessile droplets with different DsRed concentrations determine the interaction as hydrophilic one. The photoluminescence emission of dehydrated DsRed droplets is found to be slightly red-shifted, peaking at 590 nm.

Mots clés: Drops; Physico-chemical analysis; Fluorescence; Nanotechnology; Characterization; Physico-chemical characterization; Structural stabilities; Red fluorescent proteins; Thin SiO2 layers; Proteins; DsRed; Photoluminescence emission; Adsorption properties; Stability; Chemical analysis; Thin SiO₂ layers; Recombinant proteins; Cytology

Domaines: Sciences de l'ingénieur [physics]; Physique [physics]; Génie chimique

Marvine Soumbo, Alessandro Pugliara, Marie-Carmen Monje, Christine Roques, Bernard Despax, et al.. Physico-chemical characterization of the interaction of red fluorescent protein — DsRed with silica layers. 2015 IEEE Nanotechnology Materials and Devices Conference (NMDC), Sep 2015, Anchorage, United States. pp.1-2, ⟨10.1109/NMDC.2015.7439280⟩. ⟨hal-01763539⟩