Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies

Pierre Gantner (1) , Adeline Mélard (2) , Florence Damond (3) , Constance Delaugerre (4) , Julia Dina (5) , Marie Gueudin (6) , Anne Maillard (7) , Karine Sandres-Sauné (8) , Audrey Rodallec (9) , Edouard Tuaillon (10, 11) , Jean-Christophe Plantier (12) , Christine Rouzioux (13) , Véronique Avettand-Fenoel (13)

1 Laboratoire de Virologie [Strasbourg]
2 UPD5 Médecine - Université Paris Descartes - Faculté de Médecine
3 IAME (UMR_S_1137 / U1137) - Infection, Anti-microbiens, Modélisation, Evolution
4 Laboratoire de Virologie [CHU Saint-Louis]
5 U2RM - Unité de Recherche Risques Microbiens
6 Laboratoire de virologie [Rouen]
7 Centre Hospitalier Universitaire de Rennes [CHU Rennes] = Rennes University Hospital [Ponchaillou]
8 CIC 1436 - Centre d'investigation clinique de Toulouse
9 Hôpital de l'Hôtel Dieu [CHU-HCL]
10 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
11 Pathogénèse et contrôle des infections chroniques (PCCI)
12 CHU Rouen
13 Laboratoire de Virologie [CHU Necker]

Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter-laboratory reproducibility of total HIV-1-DNA quantification. METHODS: Ten laboratories of the ANRS-AC11 working group participated by quantifying HIV-DNA with a real-time qPCR assay (Biocentric) in four samples (QCMD). RESULTS: Good reproducibility was found between laboratories (standard deviation <= 0.2 log10copies/106PBMC) for the three positive QC that were correctly classified by each laboratory (QC1\textlessQC2\textlessQC3). CONCLUSIONS: Results of this QC validate the feasibility of multicenter studies using this standardized assay.

Mots clés: HIV-1 DNA; interlaboratory quality control; quantification

Domaines: Sciences du Vivant [q-bio]

Pierre Gantner, Adeline Mélard, Florence Damond, Constance Delaugerre, Julia Dina, et al.. Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies. Journal of Medical Virology, 2017, 89 (11), pp.2047--2050. ⟨10.1002/jmv.24874⟩. ⟨hal-01760708⟩