M. Clark and L. Peck, HSP70 heat shock proteins and environmental stress in Antarctic marine organisms: A mini-review, Marine Genomics, vol.2, issue.1, pp.11-19, 2009.
DOI : 10.1016/j.margen.2009.03.003

M. Meredith and J. King, Rapid climate change in the ocean west of the Antarctic Peninsula during the second half of the 20th century, Geophysical Research Letters, vol.60, issue.C10, p.19604, 2005.
DOI : 10.1023/A:1026021217991

A. Clarke, E. Murphy, M. Meredith, J. King, L. Peck et al., Climate change and the marine ecosystem of the western Antarctic Peninsula, Philosophical Transactions of the Royal Society B: Biological Sciences, vol.302, issue.5650, pp.149-66, 2007.
DOI : 10.1126/science.1090110

L. Peck, S. Morley, and M. Clark, Poor acclimation capacities in Antarctic marine ectotherms, Marine Biology, vol.82, issue.8, pp.2051-2060, 2010.
DOI : 10.1007/BF00237957

A. Bers, F. Momo, I. Schloss, and D. Abele, Analysis of trends and sudden changes in long-term environmental data from King George Island (Antarctica): relationships between global climatic oscillations and local system response, Climatic Change, vol.14, issue.7, pp.789-803, 2013.
DOI : 10.1007/978-0-387-87458-6

C. Cárdenas, M. González?aravena, and P. Santibañez, Supplemental Information 9: R code for Generalized Additive model and method of finite differences, PeerJ, vol.30, issue.3, 2018.
DOI : 10.7717/peerj.4289/supp-9

V. Fuentes, G. Alurralde, B. Meyer, G. Aguirre, A. Canepa et al., Glacial melting: an overlooked threat to Antarctic krill. Sci Rep, 2016.
DOI : 10.1038/srep27234
URL : http://www.nature.com/articles/srep27234.pdf

L. Peck, Prospects for surviving climate change in Antarctic aquatic spe? cies, Front Zool, vol.2, pp.1-8, 2005.

M. Hernandez, F. Buckle, C. Guisado, B. Barón, and N. Estavillo, Critical thermal maximum and osmotic pressure of the red sea urchin Strongylocentrotus franciscanus acclimated at different temperatures, Journal of Thermal Biology, vol.29, issue.4-5, pp.231-237, 2004.
DOI : 10.1016/j.jtherbio.2004.03.003

L. Peck, Organisms and responses to environmental change, Marine Genomics, vol.4, issue.4, pp.237-280, 2011.
DOI : 10.1016/j.margen.2011.07.001

M. Clark, K. Fraser, and L. Peck, Lack of an HSP70 heat shock response in two Antarctic marine invertebrates, Polar Biology, vol.34, issue.9, pp.1059-65, 2008.
DOI : 10.1152/ajpregu.00223.2004

M. Clark, K. Fraser, and L. Peck, Antarctic marine molluscs do have an HSP70 heat shock response, Cell Stress and Chaperones, vol.72, issue.1, pp.39-49, 2008.
DOI : 10.1152/ajpregu.00223.2004
URL : http://europepmc.org/articles/pmc2666218?pdf=render

S. Koenigstein, K. Pöhlmann, C. Held, and D. Abele, Ecological comparison of cellular stress responses among populations ??? normalizing RT-qPCR values to investigate differential environmental adaptations, BMC Ecology, vol.13, issue.1, p.21, 2013.
DOI : 10.1007/s12192-008-0015-7

T. Brey, J. Pearse, L. Basch, J. Mcclintock, and M. Slattery, Growth and production of Sterechinus neumayeri (Echinoidea: Echinodermata) in McMurdo Sound, Antarctica, Marine Biology, vol.9, issue.2, pp.279-92, 1995.
DOI : 10.1007/BF00347132

F. Ovando, C. Gimpel, C. Cardenas, J. Silva, D. Lorgeril et al., ), Journal of Shellfish Research, vol.31, issue.3, pp.875-83, 2012.
DOI : 10.2983/035.031.0336

A. Pinsino and V. Matranga, Sea urchin immune cells as sentinels of environmental stress, Developmental & Comparative Immunology, vol.49, issue.1, pp.198-205, 2015.
DOI : 10.1016/j.dci.2014.11.013

V. Matranga, G. Toia, R. Bonaventura, and W. Muller, Cellular and biochemical responses to environmental and experimentally induced stress in sea urchin coelomocytes, Cell Stress & Chaperones, vol.16, issue.2, pp.113-133, 2000.
DOI : 10.1379/1466-1268(2000)005<0113:CABRTE>2.0.CO;2
URL : http://europepmc.org/articles/pmc312897?pdf=render

D. Andrea?winslow, L. Novitski, and A. , red spherule coelomocytes after disruption of acto-myosin contractility, Integrative Zoology, vol.5, issue.2, pp.115-137, 2008.
DOI : 10.1111/j.1749-4877.2008.00086.x

A. Pinsino, D. Torre, C. Sammarini, V. Bonaventura, R. Amato et al., Sea urchin coelomocytes as a novel cellular biosensor of environmental stress: a field study in the Tremiti Island Marine Protected Area, Southern Adriatic Sea, Italy, Cell Biology and Toxicology, vol.50, issue.06, pp.541-52, 2008.
DOI : 10.1007/s10565-008-9055-0

J. Borges, P. Branco, L. Pressinotti, D. Severino, and J. Silva, Intranuclear crystalloids of Antarctic sea urchins as a biomarker for oil contamination, Polar Biology, vol.146, issue.6, pp.843-852, 2010.
DOI : 10.3184/007967401103165262

M. Lamare, M. Barker, and M. Lesser, In situ rates of DNA damage and abnormal development in Antarctic and non-Antarctic sea urchin embryos, Aquatic Biology, vol.1, issue.1, pp.21-32, 2007.
DOI : 10.3354/ab00003

D. Cowart, P. Ulrich, D. Miller, and A. Marsh, Salinity sensitivity of early embryos of the Antarctic sea urchin, Sterechinus neumayeri, Polar Biology, vol.3, issue.4, pp.435-476, 2009.
DOI : 10.1093/icb/31.1.65

J. Ericson, M. Lamare, S. Morley, and M. Barker, The response of two ecologically important Antarctic invertebrates (Sterechinus neumayeri and Parborlasia corrugatus) to reduced seawater pH: effects on fertilisation and embryonic development, Marine Biology, vol.28, issue.3, pp.2689-702, 2010.
DOI : 10.1093/icb/31.1.65

J. Ericson, M. Ho, A. Miskelly, C. King, P. Virtue et al., Combined effects of two ocean change stressors, warming and acidification, on fertilization and early development of the Antarctic echinoid Sterechinus neumayeri, Polar Biology, vol.77, issue.7, pp.1027-1061, 2012.
DOI : 10.1007/BF00395816

M. Byrne, M. Ho, L. Koleits, C. Price, C. King et al., to near-future ocean acidification and warming, Global Change Biology, vol.65, issue.7, pp.2264-75, 2013.
DOI : 10.1371/journal.pone.0052448

C. Suckling, M. Clark, J. Richard, S. Morley, M. Thorne et al., Adult acclimation to combined temperature and pH stressors significantly enhances reproductive outcomes compared to short-term exposures, Journal of Animal Ecology, vol.8, issue.111, pp.773-84, 2015.
DOI : 10.1371/journal.pone.0052448
URL : https://hal.archives-ouvertes.fr/hal-01102888

P. Branco, L. Pressinotti, J. Borges, R. Iunes, J. Kfoury?jr et al., Cellular biomarkers to elucidate global warming effects on Antarctic sea urchin Sterechinus neumayeri, Polar Biology, vol.457, issue.1, pp.221-230, 2012.
DOI : 10.1016/S0167-9309(07)80073-1

K. González, J. Gaitán?espitia, A. Font, C. Cárdenas, and M. González?aravena, Expression pattern of heat shock proteins during acute thermal stress in the Antarctic sea urchin, Sterechinus neumayeri, Revista Chilena de Historia Natural, vol.8, issue.4, pp.2-9, 2016.
DOI : 10.1371/journal.pone.0052448

E. Bachere, D. Chagot, and H. Grizel, Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutria? tion, Dev Comp Immunol, issue.12, pp.549-59, 1988.

K. Tamura, J. Dudley, M. Nei, and S. Kumar, MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0, Molecular Biology and Evolution, vol.24, issue.8, pp.1596-1605, 2007.
DOI : 10.1093/molbev/msm092
URL : https://academic.oup.com/mbe/article-pdf/24/8/1596/3858330/msm092.pdf

M. Pfaffl, A. Tichopad, C. Prgomet, and T. Neuvians, Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper ??? Excel-based tool using pair-wise correlations, Biotechnology Letters, vol.26, issue.6, pp.509-524, 2004.
DOI : 10.1023/B:BILE.0000019559.84305.47

K. Livak and T. Schmittgen, Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2???????CT Method, Methods, vol.25, issue.4, pp.402-410, 2001.
DOI : 10.1006/meth.2001.1262

J. Zar, Biostatistical analysis. New Jersey: Prentice?Hall International, 1999.

J. Bethke, V. Rojas, J. Berendsen, C. Cárdenas, F. Guzmán et al., Development of a new antibody for detecting natural killer enhancing factor (NKEF)-like protein in infected salmonids, Journal of Fish Diseases, vol.25, issue.5, pp.379-88, 2012.
DOI : 10.1016/S0145-305X(00)00038-0

M. Mayer and B. Bukau, Hsp70 chaperones: Cellular functions and molecular mechanism, Cellular and Molecular Life Sciences, vol.11, issue.Suppl.4, pp.670-84, 2005.
DOI : 10.1128/MCB.19.6.4535
URL : https://link.springer.com/content/pdf/10.1007%2Fs00018-004-4464-6.pdf

A. Knowlton and M. Salfity, Nuclear localization and the heat shock proteins, Journal of Biosciences, vol.45, issue.2, pp.123-155, 1996.
DOI : 10.1128/MCB.14.8.5088
URL : http://www.ias.ac.in/jarch/jbiosci/21/123-132.pdf

A. Piano, S. Franzellitti, F. Tinti, and E. Fabbri, Sequencing and expression pattern of inducible heat shock gene products in the European flat oyster, Ostrea edulis, Gene, vol.361, pp.119-145, 2005.
DOI : 10.1016/j.gene.2005.06.034

M. Clark, M. Thorne, J. Toullec, Y. Meng, L. Guan et al., Antarctic Krill 454 Pyrosequencing Reveals Chaperone and Stress Transcriptome, PLoS ONE, vol.340, issue.3, p.15919, 2011.
DOI : 10.1371/journal.pone.0015919.s008
URL : https://hal.archives-ouvertes.fr/hal-01250924

D. Whitley, S. Goldberg, and W. Jordan, Heat shock proteins: A review of the molecular chaperones, Journal of Vascular Surgery, vol.29, issue.4, pp.748-51, 1999.
DOI : 10.1016/S0741-5214(99)70329-0

M. Feder and G. Hofmann, HEAT-SHOCK PROTEINS, MOLECULAR CHAPERONES, AND THE STRESS RESPONSE: Evolutionary and Ecological Physiology, Annual Review of Physiology, vol.61, issue.1, pp.243-82, 1999.
DOI : 10.1146/annurev.physiol.61.1.243

L. Peck, K. Webb, and D. Bailey, Extreme sensitivity of biological function to temperature in Antarctic marine species, Functional Ecology, vol.156, issue.5, pp.625-655, 2004.
DOI : 10.1007/s00300-003-0548-2

G. Hofmann, B. Buckley, S. Airaksinen, J. Keen, and G. Somero, Heat shock protein expression is absent in the Antarctic fish Trematomus bernacchii (Family Nototheniidae), J Exp Biol, vol.203, pp.2331-2340, 2000.

A. Pockley, Heat shock proteins as regulators of the immune response, The Lancet, vol.362, issue.9382, pp.469-76, 2003.
DOI : 10.1016/S0140-6736(03)14075-5

R. Roberts, C. Agius, C. Saliba, P. Bossier, and Y. Sung, Heat shock proteins (chaperones) in fish and shellfish and their potential role in relation to fish health: a review, Journal of Fish Diseases, vol.12, issue.10, pp.789-801, 2010.
DOI : 10.1897/1551-5028(2002)021<0557:EOHOHS>2.0.CO;2