ASSOCIATION OF PO4 LOADED POROUS SILICON MICROPARTICLES WITH NACRE PROTEINS ENHANCES MINERALIZED BONE FORMATION IN VIVO

Pierre-Yves Collart Dutilleul (1) , Alban Desoutter (1) , E. Cueto-Diaz , N. Fatima , T. Gerecsei , Csilla Gergely (2) , J.-L. Montero , V. Montero , Frédérique Cunin (3) , Frédéric Cuisinier

1 LBN - Laboratoire de Bioingénierie et NanoSciences
2 L2C - Laboratoire Charles Coulomb
3 ICGM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier

We investigated bone regeneration in vitro and in vivo, using porous silicon (pSi) microparticles coupled with nacre proteins. We prepared a composite powder formed by pSi particles loaded with phosphate, and nacre proteins carrying calcium. For in vitro experiments, we used primary culture of human Dental Pulp Stem Cells. Results showed efficiency for both cell adhesion, cell differentiation and CaPO4 formation. But the most interesting results were that this composite was highly efficient to regenerate bone in vivo, even without any additional stem cells grafting.

Domaines: Biophysique [physics.bio-ph]

Pierre-Yves Collart Dutilleul, Alban Desoutter, E. Cueto-Diaz, N. Fatima, T. Gerecsei, et al.. ASSOCIATION OF PO4 LOADED POROUS SILICON MICROPARTICLES WITH NACRE PROTEINS ENHANCES MINERALIZED BONE FORMATION IN VIVO. Porous Semiconductors - Science and Technology 2018, Mar 2018, La Grande Motte, Montpellier, France. ⟨hal-01745997⟩