Skip to Main content Skip to Navigation
Journal articles

Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels

Abstract : Conspectus Many enzymes that produce or transform small molecules such as O 2 , H 2 , and CO 2 embed inorganic cofactors based on transition metals. The active site, where the chemical reaction occurs, is buried in and protected by the protein matrix, and connected to the solvent in several ways: chains of redox cofactors mediate long range electron transfer; static or dynamic tunnels guide the substrate, product and inhibitors; amino acids and water molecules transfer protons. The catalytic mechanism of these enzymes is therefore delocalised over the protein and involves many different steps, some of which determine the response of the enzyme under conditions of stress (extreme redox conditions, presence of inhibitors, light), the catalytic rates in the two directions of the reaction, and their ratio (the "catalytic bias"). Understanding all the steps in the catalytic cycle-including those that occur on sites of the protein that are remote from the active site-requires a combination of biochemical, structural, spectroscopic, theoretical and kinetic methods. Here we argue that kinetics should be used to the 1
Document type :
Journal articles
Complete list of metadatas

Cited literature [39 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01745738
Contributor : Laure Azzopardi <>
Submitted on : Friday, April 19, 2019 - 3:21:35 PM
Last modification on : Monday, December 30, 2019 - 12:04:04 AM

File

main HAL 01745738.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Melisa del Barrio, Matteo Sensi, Christophe Orain, Carole Baffert, Sébastien Dementin, et al.. Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels. Accounts of Chemical Research, American Chemical Society, 2018, 51 (3), pp.769 - 777. ⟨10.1021/acs.accounts.7b00622⟩. ⟨hal-01745738⟩

Share

Metrics

Record views

111

Files downloads

176