Alteration of the characteristics of extracellular polymeric substances (EPS) extracted from the fungus Phanerochaete chrysosporium when exposed to sub-toxic concentrations of nickel (II)

Abstract : The fungus Phanerochaete chrysosporium was incubated at five sub-toxic concentrations of Ni2+ (0.5, 1, 5, 10 and 25 mg/L, respectively), and its metal immobilization ability as well as the alteration of some characteristics regarding the extracellular polymeric substances (EPS) were investigated. With the increased Ni2+ concentrations in the broth, higher Ni2+ amounts were measured in both intact fungal cells (biomass before EPS extraction) and EPS-free biomass (biomass after EPS extraction). The Ni2+ immobilization ability of the extracted EPS displayed a similar level at Ni2+ concentrations higher than 1 mg/L. The presence of Ni2+ in the broth decreased the zeta-potential of the intact biomass and increased cell surface hydrophobicity (CSH). Fourier transform infrared spectroscopy (FT-IR) analyses identified the presence of some functional groups, such as carboxyl, phosphoryl and hydroxyl groups, in the extracted EPS. The high hydrophobicity (>60%) of the extracted EPS was decreased by the increased Ni2+ concentration. The protein (PN) and polysaccharide (PS) content in the EPS was also affected by the increased Ni2+ concentration, and the abundance of PN-like molecules ranging from 0.5 kDa to 14 kDa was enriched. However, the fluorescence characteristics and apparent molecular weight (aMW) of the extracted EPS were not affected by the Ni2+ concentration. Therefore, one possible defense mechanism developed by the fungus towards Ni2+ stress is the adjustment of its EPS composition.
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International Biodeterioration and Biodegradation, Elsevier, 2018, 〈10.1016/j.ibiod.2018.02.009〉
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https://hal.archives-ouvertes.fr/hal-01734772
Contributeur : Yoan Pechaud <>
Soumis le : jeudi 15 mars 2018 - 09:17:10
Dernière modification le : mercredi 12 décembre 2018 - 15:32:59

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Feishu Cao, Isabelle Bourven, Gilles Guibaud, Eldon Rene, Piet N.L. Lens, et al.. Alteration of the characteristics of extracellular polymeric substances (EPS) extracted from the fungus Phanerochaete chrysosporium when exposed to sub-toxic concentrations of nickel (II). International Biodeterioration and Biodegradation, Elsevier, 2018, 〈10.1016/j.ibiod.2018.02.009〉. 〈hal-01734772〉

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