MALDI-TOF MS identification of Anopheles gambiae Giles blood meal crushed on Whatman filter papers

Abstract : Background: Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens are crushed on Whatman filter papers to determine the host feeding patterns by identifying the origin of their blood meals. The aim of this study was to test whether crushing engorged mosquito abdomens on Whatman filter papers was compatible with MALDI-TOF MS for mosquito blood meal identification. Both laboratory reared and field collected mosquitoes were tested. Material and methods: Sixty Anopheles gambiae Giles were experimentally engorged on the blood of six distinct vertebrate hosts (human, sheep, rabbit, dog, chicken and rat). The engorged mosquito abdomens were crushed on Whatman filter papers for MALDI-TOF MS analysis. 150 Whatman filter papers, with mosquitoes engorged on cow and goat blood, were preserved. A total of 77 engorged mosquito abdomens collected in the Comoros Islands and crushed on Whatman filter papers were tested with MALDI-TOF MS. Results The MS profiles generated from mosquito engorged abdomens crushed on Whatman filter papers exhibited high reproducibility according to the original host blood. The blood meal host was correctly identified from mosquito abdomens crushed on Whatman filter papers by MALDI-TOF MS. The MS spectra obtained after storage were stable regardless of the room temperature and whether or not they were frozen. The MS profiles were reproducible for up to three months. For the Comoros samples, 70/77 quality MS spectra were obtained and matched with human blood spectra. This was confirmed by molecular tools. Conclusion: The results demonstrated that MALDI-TOF MS could identify mosquito blood meals from Whatman filter papers collected in the field during entomological surveys. The application of MALDI-TOF MS has proved to be rapid and successful, making it a new and efficient tool for mosquito-borne disease surveillance.
Liste complète des métadonnées

Littérature citée [39 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01730963
Contributeur : Isabelle Combe <>
Soumis le : mardi 13 mars 2018 - 18:03:27
Dernière modification le : jeudi 15 mars 2018 - 10:23:34
Document(s) archivé(s) le : jeudi 14 juin 2018 - 17:08:32

Fichier

journal.pone.0183238.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Sirama Niare, Lionel Almeras, Fatalmoudou Tandina, Amina Yssouf, Affane Bacar, et al.. MALDI-TOF MS identification of Anopheles gambiae Giles blood meal crushed on Whatman filter papers. PLoS ONE, Public Library of Science, 2017, 12 (8), pp.e0183238. 〈10.1371/journal.pone.0183238〉. 〈hal-01730963〉

Partager

Métriques

Consultations de la notice

141

Téléchargements de fichiers

19