Pore-forming activity of the $Pseudomonas\ aeruginosa$ type III secretion system translocon alters the host epigenome - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Microbiology Année : 2018

Pore-forming activity of the $Pseudomonas\ aeruginosa$ type III secretion system translocon alters the host epigenome

Résumé

Recent studies highlight that bacterial pathogens can reprogram target cells by influencing epigenetic factors. The type III secretion system (T3SS) is a bacterial nanomachine that resembles a syringe on the bacterial surface. The T3SS 'needle' delivers translocon proteins into eukaryotic cell membranes, subsequently allowing injection of bacterial effectors into the cytosol. Here we show that $Pseudomonas\ aeruginosa$ induces early T3SS-dependent dephosphorylation and deacetylation of histone H3 in eukaryotic cells. This is not triggered by any of the $P.\ aeruginosa$ T3SS effectors, but results from the insertion of the PopB-PopD translocon into the membrane. This suggests that the $P.\ aeruginosa$ translocon is a genuine T3SS effector acting as a pore-forming toxin. We visualized the translocon plugged into the host cell membrane after the bacterium has left the site of contact, and demonstrate that subsequent ion exchange through this pore is responsible for histone H3 modifications and host cell subversion.
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Dates et versions

hal-01726153 , version 1 (08-03-2018)

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Laurent Dortet, Charlotte Lombardi, François Cretin, Andréa Dessen, Alain Filloux. Pore-forming activity of the $Pseudomonas\ aeruginosa$ type III secretion system translocon alters the host epigenome. Nature Microbiology, 2018, 3, pp.378 - 386. ⟨10.1038/s41564-018-0109-7⟩. ⟨hal-01726153⟩
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