Skip to Main content Skip to Navigation
Journal articles

Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds.

Abstract : The intermediate seed category was defined in the early 1990s using coffee (Coffea arabica) as a model. In contrast to orthodox seeds, intermediate seeds cannot survive complete drying, which is a major constraint for seed storage, for both biodiversity conservation and agricultural purposes. However, intermediate seeds are considerably more tolerant to drying than recalcitrant seeds, which are highly sensitive to desiccation. To gain insight into the mechanisms governing such differences, changes in desiccation tolerance (DT), hormone content and the transcriptome were analysed in developing coffee seeds. Acquisition of DT coincided with a dramatic transcriptional switch characterised by the repression of primary metabolism, photosynthesis and respiration, and the upregulation of genes coding for late embryogenesis abundant (LEA) proteins, heat shock proteins (HSP) and antioxidant enzymes. Analysis of heat-stable proteome in the mature coffee seed confirmed the accumulation of LEA proteins identified at the transcript level. Transcriptome analysis also suggests a major role for ABA and for the transcription factors CaHSFA9, CaDREB2G, CaANAC029, CaPLATZ and CaDOG-like in DT acquisition. The ability of CaHSFA9 and CaDREB2G to trigger HSP gene transcription was validated by Agrobacterium-mediated transformation of coffee somatic embryos.
Document type :
Journal articles
Complete list of metadata

Cited literature [105 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01720116
Contributor : Archive Ouverte ProdInra Connect in order to contact the contributor
Submitted on : Tuesday, May 26, 2020 - 2:03:53 PM
Last modification on : Friday, August 5, 2022 - 10:51:03 AM

File

2018_Dussert_J Exp Bot_1.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Stéphane Dussert, Julien Serret, Aldecinei Bastos-Siqueira, Fabienne Morcillo, Eveline Déchamp, et al.. Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds.. Journal of Experimental Botany, Oxford University Press (OUP), 2018, 69 (7), pp.1583-1597. ⟨10.1093/jxb/erx492⟩. ⟨hal-01720116⟩

Share

Metrics

Record views

170

Files downloads

23