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S. Figure, Related to Figure 6. Depolarization increases the ectopic expression of AIS components in axons of MYPT mutants

E. Shrna-knockdown, KD) of MYPT2 resulted in elevated axonal pMLC (red) and AnkG (magenta) in neurons at baseline and a further increase after 60 minutes of depolarization. An shRNA to a scrambled sequence (shScr) served as a control

F. Pmlc, AnkG are elevated in the axon at baseline and further increased after depolarization in shMYPT2 knockdown (Cre; gray) but not in shScr control (EGFP; black) neurons. Depolarization resulted in an additional 1.6-fold increase of pMLC (p=0.0003) and a 1.6-fold increase of AnkG (p=0.004) relative to non-depolarized knockdown neurons. Graphs show average intensity ± SEM. Kruskal-Wallis test with Dunn's correction