Exploratory GC/MS-Based Metabolomics of Body Fluids

Carole Migné (1, 2) , Stéphanie Durand (1, 2) , Estelle Pujos-Guillot (1, 2)

1 UNH - Unité de Nutrition Humaine
2 PFEM - Plateforme Exploration du Métabolisme

GC/MS-based metabolomics is a powerful tool for metabolic phenotyping and biomarker discovery from body biofluids. In this chapter, we describe an untargeted metabolomic approach for plasma/serum and fecal water sample profiling. It describes a multistep procedure, from sample preparation, oximation/silylation derivatization, and data acquisition using GC/QToF to data processing consisting in data extraction and identification of metabolites.

Mots clés: Untargeted metabolomics; Mass spectrometry; Data processing; gas chromatography; mass spectrum analysis

Mots clés: chromatographie en phase gazeuse; spectrométrie de masse

Domaines: Chimie analytique

Carole Migné, Stéphanie Durand, Estelle Pujos-Guillot. Exploratory GC/MS-Based Metabolomics of Body Fluids. Clinical Metabolomics : Methods and Protocols, Chapter 16, Springer, 384 p., 2018, Methods in Molecular Biology, 978-1-4939-7592-4. ⟨10.1007/978-1-4939-7592-1_16⟩. ⟨hal-01699017⟩