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Preferential expression of domain cassettes 4, 8 and 13 of Plasmodium falciparum erythrocyte membrane protein 1 in severe malaria imported in France

S Argy S Bertin S Milet P. Hubert 1 S. Clain 2 S. Cojean S. Houze 3 S Tuikue-Ndam P Kendjo S Deloron N. Argy 3 G. Bertin 4 J. Milet V. Hubert J. Clain P. Houzé N. Tuikue-Ndam E. Kendjo 3 P. Deloron 5 S. Matheron E. Casalino M. Wolff 6 A. Delaval P. Agnamey R. Durand 7 E. Pilo C. Rapp F. Faucher B. Cuisenier 8 I. Poilane D. Bemba A. Roide A. Debourgogne 9 M. Thibault D. Toubas 10 P. Patoz L. de Gentile D. Pons 11 P. Hurst C. Lohmann M. Bigel N. Godineau M. Thouvenin 12 J. Dunand N. Ait-Ammar A. Angoulvant 13 N. Dahane M. Lefevre 14 B. Murat C. Garnaud 15 E. Dannaoui 16 F. Botterel 17 E. Dutoit 18 M. Dardé H. Ichou C. Branger 19 P. Penn C. Angebault F. Morio 20 L. Bret 21 M. Thellier 22 O. Mouri 23 E. Cateau 24, 25 J.Y. Siriez O. Fenneteau M. Revest 26 S. Belaz 27 G. Belkadi 28 S. Hamane S. Bretagne 29 A. Aboubacar G. Leloup M. Develoux H. Lapillonne O. Eloy G. Nevez D. Raffenot B. Buret G. Desoubeaux 30 A. Goepp
15 TIMC-IMAG-TheREx - Thérapeutique Recombinante Expérimentale
TIMC-IMAG - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
25 MDE - Microbiologie de l'Eau
EBI - Ecologie et biologie des interactions
Abstract : Objectives - Severe Plasmodium falciparum malaria (SM) involves cytoadhesion of parasitized red blood cells, mediated by P. falciparum erythrocyte membrane protein 1, which is encoded by var genes. Expression of var gene group A and B or encoding domain cassettes DC4, DC5, DC8 and DC13 has been implicated in SM in African children, but no data exist in the context of imported malaria. The aim of this study was to investigate var gene expression linked to clinical presentation and host factors in SM imported into France. Methods - Expression level of var gene groups A, B, C, var1, var2csa, var3 and var genes encoding DC4, DC5, DC8 and DC13 was measured by quantitative RT-PCR and expressed in transcript units. Seventy SM and 48 uncomplicated malaria (UM) P. falciparum cases were analysed according to disease severity, epidemiological characteristics (migrants or travellers) and anti-P. falciparum antibodies. Cluster analysis was performed to identify gene expression profiles. Results - Var1 and B/C expression were higher in UM than SM (0.66 (0-1.1) and 1.88 (1.3-2.4); p <0.04, respectively). Group C expression differed between migrants and travellers (0.21 (0-0.75) versus 0 (0-0); p 0.002). Group A differed in naive and pre-exposed patients (1.1 (0.7-1.5) versus 0.4 (0-1.1); p 0.01). Population clusters revealed increased expression from group A and B var genes, and DC4, DC8 and DC13 in SM. Conclusions - These results corroborate the implication of DC4, DC8 and DC13 in severe imported malaria cases as African children, and their expression depends of host factors.
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S Argy, S Bertin, S Milet, P. Hubert, S. Clain, et al.. Preferential expression of domain cassettes 4, 8 and 13 of Plasmodium falciparum erythrocyte membrane protein 1 in severe malaria imported in France. Clinical Microbiology and Infection, Elsevier for the European Society of Clinical Microbiology and Infectious Diseases, 2017, 23 (3), pp.211.e1 - 211.e4. ⟨10.1016/j.cmi.2016.10.012⟩. ⟨hal-01698251⟩



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