Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

The inhibition of TOR in the model diatom Phaeodactylum tricornutum promotes a get-fat growth regime

Abstract : The target of rapamycin (TOR) signaling pathway regulates fundamental intracellular functions critical for cell viability and proliferation. Manipulation of TOR in high lipid-producing microalgae may help overcome the trade-off between biomass production and lipid yield that still impairs the viable production of biofuel from microalgae. In this study, we inhibited the TOR kinase in the model diatom Phaeodactylum tricornutum using the selective TOR inhibitor AZD-8055, and analyzed cell proliferation, chlorophyll content, lipid synthesisand carbon metabolism. AZD-8055 inhibits cell proliferation in a dose-dependent manner compared to N deprivation which stops growth. Microscopy, flow cytometry, and quantitative analyses of lipids also demonstrated that AZD-8055 treatment strongly promotes triacylglycerol (TAG) accumulation while decreasing the quantity of sterols. The TAG productivity of AZD-8055 treated cultures was significantly higher than for N deprived cultures. Measurement of the activities of the key metabolic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH), glucose-6-phosphate dehydrogenase (G6PDH) and malate dehydrogenase (MDH) revealed opposite effects for AZD-8055 treatment and N-starvation on the activity of the glycolytic enzyme GAPDH. This suggests that TOR inhibition and N starvation may have distinct impacts on general metabolism and lipid accumulation. Our main finding is that treating cultures with AZD-8055 results in higher TAG productivity than N starvation in P. tricornutum. The chemical or genetic manipulation of the TOR signaling pathway in P. tricornutum and other diatoms may lead to the development of strains or approaches suitable for the enhanced production of TAGs for biofuel.
Document type :
Journal articles
Complete list of metadata

Cited literature [47 references]  Display  Hide  Download
Contributor : LAURE AZZOPARDI Connect in order to contact the contributor
Submitted on : Tuesday, April 24, 2018 - 4:31:14 PM
Last modification on : Saturday, June 25, 2022 - 8:28:21 PM
Long-term archiving on: : Wednesday, September 19, 2018 - 2:30:07 PM


01696878 Prioretti et al.pdf
Files produced by the author(s)



Laura Prioretti, Luisana Avilan, Fredéric Carrière, Marie-Helene Montane, Ben Field, et al.. The inhibition of TOR in the model diatom Phaeodactylum tricornutum promotes a get-fat growth regime. Algal Research - Biomass, Biofuels and Bioproducts, Elsevier, 2017, 26, pp.265 - 274. ⟨10.1016/j.algal.2017.08.009⟩. ⟨hal-01696878⟩



Record views


Files downloads