Metaflumizone inhibits the honeybee NaV 1 channel by targeting recovery from slow inactivation.

Pascal Gosselin-Badaroudine (1) , Pierre Charnet (2) , Claude Collet (3) , Mohamed Chahine (1, 4)

1 CERVO - Centre de recherche de l’Institut universitaire en santé mentale de Québec [Canada]
2 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
3 AE - Abeilles et Environnement
4 Department of Medicine

Metaflumizone is the latest addition to the armamentarium of the Na+ channel inhibitor insecticide family. We used the Xenopus oocyte expression system and a Markovian model to assess the effect of metaflumizone on Apis mellifera Na+ channels (AmNaV 1). Our results reveal that metaflumizone inhibits AmNaV 1 channels by targeting the kinetics of recovery from slow inactivation. Multistate modeling of fast and slow inactivation of the AmNaV 1 channel made it possible to study the effects of metaflumizone on a set of rate constants underlying the transition between the open and inactivated conformations and provided insights into their specificity. We conclude that the methods we used could be extended to assessing the toxicity of other Na+ channel inhibitor insecticides.

Mots clés: AmNaV1; NaV; insecticides; metaflumizone; voltage-gated Na+ channels; bee; pollinator; membrane channel; in vitro study; modelling

Mots clés: abeille; pollinisateur; canal ionique; sodium; mode d'action; toxicologie; étude in vitro; modélisation

Domaines: Sciences du Vivant [q-bio]

Pascal Gosselin-Badaroudine, Pierre Charnet, Claude Collet, Mohamed Chahine. Metaflumizone inhibits the honeybee NaV 1 channel by targeting recovery from slow inactivation.. FEBS Letters, 2017, 591 (23), pp.3842-3849. ⟨10.1002/1873-3468.12897⟩. ⟨hal-01695891⟩