Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes

Peggy R Biga (1) , Mary N Latimer (1) , Jacob Michael Froehlich (1) , Jean-Charles Gabillard (2) , Iban Seiliez (3)

1 Department of Biology
2 LPGP - Laboratoire de Physiologie et Génomique des Poissons
3 NuMéA - Nutrition, Métabolisme, Aquaculture

The zebrafish (Danio rerio) remains the teleost fish of choice for biological investigations due to the vast array of molecular tools and resources available. To better understand the epigenetic regulation of autophagy, we utilized a primary myotube culture system generated from isolated myogenic precursor cells (MPCs) from zebrafish grown under starvation conditions using a media devoid of serum and amino acids. Here, we report starvation-induced regulation of several autophagy-related genes (atg) expression and profile the distribution of H3K27me3, H3K9me3, and H3K4me3 marks along lc3b, atg4b and p62/sqstm1 loci. These data support epigenetic regulation of autophagy in response to starvation that suggests a level of regulation that can be sustained for chronic conditions via chromatin modification.

Mots clés: zebrafish; atrophy; histone modification; autophagy; epigenetic; danio devario; fish; animal growth

Mots clés: poisson; danio rerio; cyprinidae; épigénétique; histone; régulation transcriptionnelle; stress; régime alimentaire; expression des gènes; croissance animale; autophagie; myotube; atrophie; muscle; poisson zèbre

Domaines: Sciences du Vivant [q-bio]

Peggy R Biga, Mary N Latimer, Jacob Michael Froehlich, Jean-Charles Gabillard, Iban Seiliez. Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes. Biology Open, 2017, 6 (11), pp.1720-1725. ⟨10.1242/bio.029090⟩. ⟨hal-01675818⟩