Microbial adhesion on novel yttria-stabilized tetragonal zirconia (Y-TZP) implant surfaces with nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) coatings

S. Schienle , A. Al-Ahmad , R.J. Kohal , F. Bernsmann , E. Adolfsson , L. Montanaro , P. Palmero , T. Fürderer , Jérome Chevalier (1) , E. Hellwig , L. Karygianni

1 MATEIS - Matériaux, ingénierie et science [Villeurbanne]

Objectives: Biomaterial surfaces are at high risk for initial microbial colonization, persistence, and concomitant infection. The rationale of this study was to assess the initial adhesion on novel implant surfaces of Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans upon incubation. Materials and methods: The tested samples were 3 mol% yttria-stabilized tetragonal zirconia polycrystal (3Y-TZP) samples with nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) coating (A) and 3Y-TZP samples coated with ceria-stabilized zirconia-based (Ce-TZP) composite and a-C:H:N (B). Uncoated 3Y-TZP samples (C) and bovine enamel slabs (BES) served as controls. Once the surface was characterized, the adherent microorganisms were quantified by estimating the colony-forming units (CFUs). Microbial vitality was assessed by live/dead staining, and microbial-biomaterial surface topography was visualized by scanning electron microscopy (SEM). Results: Overall, A and B presented the lowest CFU values for all microorganisms, while C sheltered significantly less E. faecalis, P. aeruginosa, and C. albicans than BES. Compared to the controls, B demonstrated the lowest vitality values for E. coli (54.12 %) and C. albicans (67.99 %). Interestingly, A (29.24 %) exhibited higher eradication rates for S. aureus than B (13.95 %). Conclusions: Within the limitations of this study, a-C:H:N-coated 3Y-TZP surfaces tended to harbor less initially adherent microorganisms and selectively interfered with their vitality. Clinical relevance: This could enable further investigation of the new multi-functional zirconia surfaces to confirm their favorable antimicrobial properties in vivo. © 2015, Springer-Verlag Berlin Heidelberg.

Mots clés: Materials Testing; Microscopy; Electron; biocompatible coated material; dental material; yttria; zirconium oxide; animal; bacterium adherence; bovine; Candida albicans; chemistry; Enterococcus faecalis; Escherichia coli; Pseudomonas aeruginosa; scanning electron microscopy; staining; Staphylococcus aureus; stem cell; Animals; Bacterial Adhesion; Carbon; Cattle; Coated Materials; Biocompatible; Dental Materials; Scanning; Nitrogen; Staining and Labeling; Stem Cells; Yttrium; Zirconium

Domaines: Matériaux

S. Schienle, A. Al-Ahmad, R.J. Kohal, F. Bernsmann, E. Adolfsson, et al.. Microbial adhesion on novel yttria-stabilized tetragonal zirconia (Y-TZP) implant surfaces with nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) coatings. Clinical Oral Investigations, 2016, 20 (7), pp.1719-1732. ⟨10.1007/s00784-015-1655-5⟩. ⟨hal-01804524⟩