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Article Dans Une Revue Biochemical Engineering Journal Année : 2018

Resveratrol production by grapevine cells in fed-batch bioreactor: Experiments and modelling

Résumé

The purpose of this work was to scale-up the culture of grapevine cells (Vitis labrusca) from shake-flasks (100 mL) to a 5L stirred bioreactor in order to develop a model able to describe the bioproduction of resveratrol under the controlled conditions of a fed-batch culture. For this study, the biomass, resveratrol and sugar concentrations as well as pH and dissolved oxygen were monitored daily. The experiments were conducted twice over a three month period. The culture was elicited during the exponential growth phase with methyl jasmonate, leading the cells to exhibit a complex behaviour during the resveratrol production phase. A model of the system behaviour involving simple mechanisms is proposed and successfully confronted to the experimental results. This model demonstrates that the system dynamic can be decomposed into four phases: a lag phase (cell growth slowing down), a starting phase (beginning of resveratrol production), a surge phase (significant resveratrol production accompanied by significant cell death) and a stationary phase. Thus, we were able to successfully scale-up resveratrol production from 100 mL flasks to 5L bioreactors.
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Dates et versions

hal-01666093 , version 1 (22-07-2020)

Identifiants

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Thomas Chastang, Victor Pozzobon, Behnam Taidi, Eric Courot, Christophe Clément, et al.. Resveratrol production by grapevine cells in fed-batch bioreactor: Experiments and modelling. Biochemical Engineering Journal, 2018, 131, pp.9 - 16. ⟨10.1016/j.bej.2017.12.009⟩. ⟨hal-01666093⟩
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