Skip to Main content Skip to Navigation

Detection of long-range electrostatic interactions between charged molecules by means of fluorescence correlation spectroscopy

Abstract : The present paper deals with an experimental feasibility study concerning the detection of long- range intermolecular interactions through molecular diffusion behavior in solution. This follows previous analyses, theoretical and numerical, where it was found that inter-biomolecular long-range force fields of electrodynamic origin could be detected through deviations from Brownian diffusion. The suggested experimental technique was Fluorescence Correlation Spectroscopy (FCS). By con- sidering two oppositely charged molecular species in watery solution, that is, Lysozyme protein and a fluorescent dye molecule (Alexa488), the diffusion coefficient of the dye has been measured by means of the FCS technique at different values of the concentration of Lysozyme molecules, that is, at different average distances between the oppositely charged molecules. For the model consid- ered long-range interactions are built-in as electrostatic forces, the action radius of which can be varied by changing the ionic strength of the solution. The experimental outcomes clearly prove the detectability of long-range intermolecular interactions by means of the FCS technique. Molecular Dynamics simulations provide a clear and unambiguous interpretation of the experimental results.
Document type :
Journal articles
Complete list of metadatas

Cited literature [26 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01662889
Contributor : Stéphanie Suciu <>
Submitted on : Tuesday, April 24, 2018 - 4:34:26 PM
Last modification on : Wednesday, January 29, 2020 - 11:10:04 AM
Document(s) archivé(s) le : Wednesday, September 19, 2018 - 8:26:17 PM

File

1705.08285.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Ilaria Nardecchia, Mathias Lechelon, Matteo Gori, Irene Donato, Jordane Preto, et al.. Detection of long-range electrostatic interactions between charged molecules by means of fluorescence correlation spectroscopy. Physical Review E , American Physical Society (APS), 2017, 96 (2), pp.022403 ⟨10.1103/PhysRevE.96.022403⟩. ⟨hal-01662889⟩

Share

Metrics

Record views

279

Files downloads

271