A. Azioune, M. Storch, M. Bornens, M. Thé-ry, and M. Piel, Simple and rapid process for single cell micro-patterning, Lab on a Chip, vol.21, issue.26, pp.1640-1642, 2009.
DOI : 10.1039/b821581m

W. M. Bement, A. L. Miller, V. Dassow, and G. , Rho GTPase activity zones and transient contractile arrays, BioEssays, vol.45, issue.10, pp.983-993, 2006.
DOI : 10.1017/CBO9780511529764

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364130/pdf

P. Bieling, T. D. Li, J. Weichsel, R. Mcgorty, P. Jreij et al., Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks, Cell, vol.164, issue.1-2, pp.115-127, 2016.
DOI : 10.1016/j.cell.2015.11.057

URL : https://doi.org/10.1016/j.cell.2015.11.057

S. Bisi, A. Disanza, C. Malinverno, E. Frittoli, A. Palamidessi et al., Membrane and actin dynamics interplay at lamellipodia leading edge, Current Opinion in Cell Biology, vol.25, issue.5, 2013.
DOI : 10.1016/j.ceb.2013.04.001

O. Bla-zevit-s, Y. G. Mideksa, M. Solman, A. Ligabue, N. Ariotti et al., Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering, Scientific Reports, vol.18, issue.1, p.24165, 2016.
DOI : 10.1016/j.ceb.2006.06.007

M. Bonny, X. Hui, J. Schweizer, L. Kaestner, A. Zeug et al., C2-domain mediated nano-cluster formation increases calcium signaling efficiency, Scientific Reports, vol.110, issue.1, p.36028, 2016.
DOI : 10.1016/j.bpj.2016.02.031

URL : http://www.nature.com/articles/srep36028.pdf

B. M. Burkel, H. A. Benink, E. M. Vaughan, G. Von-dassow, and W. M. Bement, A Rho GTPase Signal Treadmill Backs a Contractile Array, Developmental Cell, vol.23, issue.2, pp.384-396, 2012.
DOI : 10.1016/j.devcel.2012.05.025

URL : https://doi.org/10.1016/j.devcel.2012.05.025

M. Castellana, M. Z. Wilson, Y. Xu, P. Joshi, I. M. Cristea et al., Enzyme clustering accelerates processing of intermediates through metabolic channeling, Nature Biotechnology, vol.89, issue.10, pp.1011-1018, 2014.
DOI : 10.1007/s12575-009-9008-x

URL : http://www.nature.com/nbt/journal/v32/n10/pdf/nbt.3018.pdf

M. Cebecauer, M. Spitaler, A. Sergé, and A. I. Magee, Signalling complexes and clusters: functional advantages and methodological hurdles, Journal of Cell Science, vol.123, issue.3, pp.309-320, 2010.
DOI : 10.1242/jcs.061739

URL : https://hal.archives-ouvertes.fr/hal-00502965

A. Chazeau, A. Mehidi, D. Nair, J. J. Gautier, C. Leduc et al., Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion, The EMBO Journal, vol.33, issue.23, pp.2745-2764, 2014.
DOI : 10.15252/embj.201488837

URL : http://emboj.embopress.org/content/embojnl/33/23/2745.full.pdf

S. Das, T. Yin, Q. Yang, J. Zhang, Y. I. Wu et al., Single-molecule tracking of small GTPase Rac1 uncovers spatial regulation of membrane translocation and mechanism for polarized signaling, Proc. Natl. Acad. Sci, 2015.
DOI : 10.1371/journal.pone.0007724

M. J. Davis, B. H. Ha, E. C. Holman, R. Halaban, J. Schlessinger et al., RAC1P29S is a spontaneously activating cancer-associated GTPase, Proc. Natl. Acad. Sci. U S A, pp.912-917, 2013.
DOI : 10.1093/nar/gkh398

URL : http://www.pnas.org/content/110/3/912.full.pdf

N. Durisic, L. Laparra-cuervo, A. Sandoval-a-´-lvarez, J. S. Borbely, and M. Lakadamyali, Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate, Nature Methods, vol.207, issue.2, pp.156-162, 2014.
DOI : 10.1103/PhysRevB.75.125431

C. V. Finkielstein, M. Overduin, C. , and D. G. , Cell Migration and Signaling Specificity Is Determined by the Phosphatidylserine Recognition Motif of Rac1, Journal of Biological Chemistry, vol.1376, issue.37, pp.27317-27326, 2006.
DOI : 10.1038/nsb0297-147

F. Fricke, J. Beaudouin, R. Eils, and M. Heilemann, One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy, Scientific Reports, vol.10, issue.1, p.14072, 2015.
DOI : 10.1038/nmeth.2566

R. D. Fritz and O. Pertz, The dynamics of spatio-temporal Rho GTPase signaling: formation of signaling patterns, F1000Research, vol.5, 2016.
DOI : 10.12688/f1000research.7370.1

M. F. Garcia-parajo, A. Cambi, J. A. Torreno-pina, N. Thompson, and K. Jacobson, Nanoclustering as a dominant feature of plasma membrane organization, Journal of Cell Science, vol.127, issue.23, pp.4995-5005, 2014.
DOI : 10.1242/jcs.146340

J. J. Gautier, M. E. Lomakina, L. Bouslama-oueghlani, E. Derivery, H. Beilinson et al., Clathrin is required for Scar/Wave-mediated lamellipodium formation, 2011.
DOI : 10.1242/dev.075515

URL : https://hal.archives-ouvertes.fr/tel-00647325

J. B. Gc, B. S. Gerstman, R. V. Stahelin, C. , and P. P. , lipids in the plasma membrane, Phys. Chem. Chem. Phys., vol.124, issue.2, pp.7-9, 2016.
DOI : 10.1016/j.cell.2005.10.035

J. T. Groves and J. Kuriyan, Molecular mechanisms in signal transduction at the membrane, Nature Structural & Molecular Biology, vol.36, issue.6, pp.659-665, 2010.
DOI : 10.1128/MCB.18.2.880

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703790/pdf

J. F. Hancock and R. G. Parton, Ras plasma membrane signalling platforms, Biochemical Journal, vol.389, issue.1, pp.1-11, 2005.
DOI : 10.1042/BJ20050231

A. S. Harding and J. F. Hancock, Using plasma membrane nanoclusters to build better signaling circuits, Trends in Cell Biology, vol.18, issue.8, pp.364-371, 2008.
DOI : 10.1016/j.tcb.2008.05.006

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780343/pdf

J. M. Haugh, F. Codazzi, M. Teruel, M. , and T. , Spatial Sensing in Fibroblasts Mediated by 3??? Phosphoinositides, The Journal of Cell Biology, vol.15, issue.6, pp.1269-1280, 2000.
DOI : 10.1016/S0955-0674(96)80050-0

URL : http://jcb.rupress.org/content/jcb/151/6/1269.full.pdf

W. D. Heo, T. Inoue, W. S. Park, M. L. Kim, B. O. Park et al., PI(3,4,5)P3 and PI(4,5)P2 Lipids Target Proteins with Polybasic Clusters to the Plasma Membrane, Science, vol.314, issue.5804, pp.1458-1461, 2006.
DOI : 10.1126/science.1134389

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579512/pdf

G. A. Hobbs, A. Wittinghofer, and C. J. Der, Selective Targeting of the KRAS G12C Mutant: Kicking KRAS When It???s Down, Cancer Cell, vol.29, issue.3, pp.251-253, 2016.
DOI : 10.1016/j.ccell.2016.02.015

L. Hodgson, D. Spiering, M. Sabouri-ghomi, O. Dagliyan, C. Dermardirossian et al., FRET binding antenna reports spatiotemporal dynamics of GDI???Cdc42 GTPase interactions, Nature Chemical Biology, vol.114, issue.10, pp.802-809, 2016.
DOI : 10.1214/aos/1176344552

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030135/pdf

A. Honigmann, G. Van-den-bogaart, E. Iraheta, H. J. Risselada, D. Milovanovic et al., Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment, Nature Structural & Molecular Biology, vol.352, issue.6, pp.679-686, 2013.
DOI : 10.1021/ct700301q

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676452/pdf

C. Huang, M. Tang, C. Shi, P. A. Iglesias, and P. N. Devreotes, An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration, Nature Cell Biology, vol.322, issue.11, pp.1307-1316, 2013.
DOI : 10.1007/s10915-007-9174-4

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838899/pdf

G. Iyengar and M. Rao, A cellular solution to an information-processing problem, Proceedings of the National Academy of Sciences, vol.445, issue.7126, pp.12402-12407, 2014.
DOI : 10.1038/nature05464

URL : http://www.pnas.org/content/111/34/12402.full.pdf

C. Ji, Y. Zhang, P. Xu, T. Xu, L. et al., Nanoscale Landscape of Phosphoinositides Revealed by Specific Pleckstrin Homology (PH) Domains Using Single-molecule Superresolution Imaging in the Plasma Membrane, Journal of Biological Chemistry, vol.221, issue.45, pp.26978-26993, 2015.
DOI : 10.1073/pnas.1005817107

J. L. Johnson, J. W. Erickson, C. , and R. A. , C-terminal di-arginine motif of Cdc42 protein is essential for binding to phosphatidylinositol 4,5-bi- sphosphate-containing membranes and inducing cellular transformation, 2012.

M. J. Kennedy, R. M. Hughes, L. A. Peteya, J. W. Schwartz, M. D. Ehlers et al., Rapid blue-light???mediated induction of protein interactions in living cells, Nature Methods, vol.1, issue.12, pp.973-975, 2010.
DOI : 10.1038/nprot.2006.168

B. N. Kholodenko, J. F. Hancock, and W. Kolch, Signalling ballet in space and time, Nature Reviews Molecular Cell Biology, vol.3, issue.6, pp.414-426, 2010.
DOI : 10.1038/msb.2009.19

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977972/pdf

D. V. Kö-ster, K. Husain, E. Iljazi, A. Bhat, P. Bieling et al., Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer, Proc. Natl. Acad. Sci. U S A, pp.1645-1654, 2016.

M. Krause and A. Gautreau, Steering cell migration: lamellipodium dynamics and the regulation of directional persistence, Nature Reviews Molecular Cell Biology, vol.289, issue.9, pp.577-590, 2014.
DOI : 10.1074/jbc.M113.516047

C. D. Lawson and K. Burridge, The on-off relationship of Rho and Rac during integrin-mediated adhesion and cell migration, Small GTPases, vol.114, issue.1, 2014.
DOI : 10.1021/bi300758e

A. M. Lebensohn and M. W. Kirschner, Activation of the WAVE Complex by Coincident Signals Controls Actin Assembly, Molecular Cell, vol.36, issue.3, pp.512-524, 2009.
DOI : 10.1016/j.molcel.2009.10.024

L. Li, X. Shi, X. Guo, H. Li, and C. Xu, Ionic protein???lipid interaction at the plasma membrane: what can the charge do?, Trends in Biochemical Sciences, vol.39, issue.3, pp.130-140, 2014.
DOI : 10.1016/j.tibs.2014.01.002

M. Machacek, L. Hodgson, C. Welch, H. Elliott, O. Pertz et al., Coordination of Rho GTPase activities during cell protrusion, Nature, vol.406, issue.7260, pp.99-103, 2009.
DOI : 10.1091/mbc.12.9.2711

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885353/pdf

S. Manley, J. M. Gillette, G. H. Patterson, H. Shroff, H. F. Hess et al., High-density mapping of single-molecule trajectories with photoactivated localization microscopy, Nature Methods, vol.70, issue.2, pp.155-157, 2008.
DOI : 10.1083/jcb.200508165

M. C. Mendoza, M. Vilela, J. E. Juarez, J. Blenis, and G. Danuser, ERK reinforces actin polymerization to power persistent edge protrusion during motility, Science Signaling, vol.519, issue.18, p.47, 2015.
DOI : 10.1016/B978-0-12-405539-1.00009-9

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830495/pdf

Y. Moshfegh, J. J. Bravo-cordero, V. Miskolci, J. Condeelis, and L. Hodgson, A Trio???Rac1???Pak1 signalling axis drives invadopodia??disassembly, Nature Cell Biology, vol.17, issue.6, pp.574-586, 2014.
DOI : 10.1242/jcs.005298

URL : http://www.nature.com/ncb/journal/v17/n3/pdf/ncb3123.pdf

I. Navarro-lé-rida, S. Sá-nchez-perales, M. Calvo, C. Rentero, Y. Zheng et al., A palmitoylation switch mechanism regulates Rac1 function and membrane organization, The EMBO Journal, vol.273, issue.3, pp.534-551, 2012.
DOI : 10.1074/jbc.273.40.25728

D. Normanno, L. Boudarè-ne, C. Dugast-darzacq, J. Chen, C. Richter et al., Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher, Nature Communications, vol.10, p.7357, 2015.
DOI : 10.1038/nmeth.2566

T. Oikawa, H. Yamaguchi, T. Itoh, M. Kato, T. Ijuin et al., PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia, Nature Cell Biology, vol.23, issue.5, pp.420-426, 2004.
DOI : 10.1128/MCB.19.9.6286

G. E. Peng, S. R. Wilson, and O. D. Weiner, A pharmacological cocktail for arresting actin dynamics in living cells, Molecular Biology of the Cell, vol.465, issue.7296, pp.3986-3994, 2011.
DOI : 10.1038/nature08994

O. Pertz, Spatio-temporal Rho GTPase signaling - where are we now?, Journal of Cell Science, vol.123, issue.11, pp.1841-1850, 2010.
DOI : 10.1242/jcs.064345

URL : http://jcs.biologists.org/content/joces/123/11/1841.full.pdf

R. J. Petrie, A. D. Doyle, and K. M. Yamada, Random versus directionally persistent cell migration, Nature Reviews Molecular Cell Biology, vol.1, issue.8, pp.538-549, 2009.
DOI : 10.1016/S0002-9440(10)62991-4

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2752299/pdf

A. Pezzarossa, F. Zosel, and T. Schmidt, Visualization of HRas Domains in the Plasma Membrane of Fibroblasts, Biophysical Journal, vol.108, issue.8, pp.1870-1877, 2015.
DOI : 10.1016/j.bpj.2015.03.006

L. Picas, F. Gaits-iacovoni, and B. Goud, The emerging role of phosphoinositide clustering in intracellular trafficking and signal transduction, F1000Research, vol.5, 2016.
DOI : 10.12688/f1000research.7537.1

S. J. Plowman, C. Muncke, R. G. Parton, and J. F. Hancock, H-ras, K-ras, and inner plasma membrane raft proteins operate in nanoclusters with differential dependence on the actin cytoskeleton, Proc. Natl. Acad. Sci. U S A, pp.15500-15505, 2005.
DOI : 10.1042/BJ20050231

E. Roob, N. Trendel, P. Rein-ten-wolde, and A. Mugler, Cooperative Clustering Digitizes Biochemical Signaling and Enhances its Fidelity, Biophysical Journal, vol.110, issue.7, pp.1661-1669, 2016.
DOI : 10.1016/j.bpj.2016.02.031

URL : https://doi.org/10.1016/j.bpj.2016.02.031

O. Rossier, V. Octeau, J. B. Sibarita, C. Leduc, B. Tessier et al., Integrins ??1 and ??3 exhibit distinct dynamic nanoscale organizations inside focal adhesions, Nature Cell Biology, vol.27, issue.10, pp.1057-1067, 2012.
DOI : 10.1523/JNEUROSCI.3349-07.2007

URL : https://hal.archives-ouvertes.fr/hal-00909235

R. S. Salamon and J. M. Backer, Phosphatidylinositol-3,4,5-trisphosphate: Tool of choice for class I PI 3-kinases, BioEssays, vol.273, issue.7, pp.602-611, 2013.
DOI : 10.1074/jbc.273.12.7024

G. J. Sch?-utz, H. Schindler, and T. Schmidt, Single-molecule microscopy on model membranes reveals anomalous diffusion, Biophysical Journal, vol.73, issue.2, pp.1073-1080, 1997.
DOI : 10.1016/S0006-3495(97)78139-6

M. A. Schwartz, Integrins, Oncogenes, and Anchorage Independence, The Journal of Cell Biology, vol.5, issue.3, 1997.
DOI : 10.1016/0955-0674(95)80110-3

URL : http://jcb.rupress.org/content/jcb/139/3/575.full.pdf

A. C. Shibata, L. H. Chen, R. Nagai, F. Ishidate, R. Chadda et al., Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis, Cytoskeleton, vol.22, issue.3, pp.161-177, 2013.
DOI : 10.1096/fj.07-090571

A. Shivanandan, J. Unnikrishnan, and A. Radenovic, On characterizing membrane protein clusters with model-free spatial correlation approaches . bioRxiv. https, 2015.
DOI : 10.1101/030718

C. G. Specht, N. Gr?-unewald, O. Pascual, N. Rostgaard, G. Schwarz et al., Regulation of glycine receptor diffusion properties and gephyrin interactions by protein kinase C, The EMBO Journal, vol.26, issue.18, pp.3842-3853, 2011.
DOI : 10.1038/sj.emboj.7601625

R. V. Stahelin, J. L. Scott, and C. T. Frick, Cellular and molecular interactions of phosphoinositides and peripheral proteins, Chemistry and Physics of Lipids, vol.182, pp.3-18, 2014.
DOI : 10.1016/j.chemphyslip.2014.02.002

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4484752/pdf

M. Thé-ry, V. Racine, M. Piel, A. Pé-pin, A. Dimitrov et al., Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity, Proc. Natl. Acad, 2006.

T. Tian, A. Harding, K. Inder, S. Plowman, R. G. Parton et al., Plasma membrane nanoswitches generate high-fidelity Ras signal transduction, Nature Cell Biology, vol.14, issue.8, pp.905-914, 2007.
DOI : 10.1046/j.1365-2818.2003.01093.x

T. N. Tran, K. Drab, and M. Daszykowski, Revised DBSCAN algorithm to cluster data with dense adjacent clusters, Chemometrics and Intelligent Laboratory Systems, vol.120, pp.92-96, 2013.
DOI : 10.1016/j.chemolab.2012.11.006

K. Um, S. Niu, J. G. Duman, J. X. Cheng, Y. K. Tu et al., Dynamic Control of Excitatory Synapse Development by a Rac1 GEF/GAP Regulatory Complex, Developmental Cell, vol.29, issue.6, pp.701-715, 2014.
DOI : 10.1016/j.devcel.2014.05.011

L. Valon, F. Etoc, A. Remorino, F. Di-pietro, X. Morin et al., Predictive Spatiotemporal Manipulation of Signaling Perturbations Using Optogenetics, Biophysical Journal, vol.109, issue.9, pp.1785-1797, 2015.
DOI : 10.1016/j.bpj.2015.08.042

URL : https://doi.org/10.1016/j.bpj.2015.08.042

G. Van-den-bogaart, K. Meyenberg, H. J. Risselada, H. Amin, K. I. Willig et al., Membrane protein sequestering by ionic protein???lipid interactions, Nature, vol.387, issue.7374, pp.552-555, 2011.
DOI : 10.1038/42408

S. L. Veatch, B. B. Machta, S. A. Shelby, E. N. Chiang, D. A. Holowka et al., Correlation Functions Quantify Super-Resolution Images and Estimate Apparent Clustering Due to Over-Counting, PLoS ONE, vol.37, issue.2, 2012.
DOI : 10.1371/journal.pone.0031457.s001

URL : https://doi.org/10.1371/journal.pone.0031457

J. Viaud, F. Lagarrigue, D. Ramel, S. Allart, G. Chicanne et al., Phosphatidylinositol 5-phosphate regulates invasion through binding and activation of Tiam1, Nature Communications, vol.63, p.4080, 2014.
DOI : 10.1016/S0091-679X(01)63033-4

URL : http://www.nature.com/articles/ncomms5080.pdf

J. Wang and D. A. Richards, Segregation of PIP2 and PIP3 into distinct nanoscale regions within the plasma membrane, Biology Open, vol.1, issue.9, pp.857-862, 2012.
DOI : 10.1242/bio.20122071

S. Wang, T. Watanabe, K. Matsuzawa, A. Katsumi, M. Kakeno et al., Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration, The Journal of Cell Biology, vol.199, issue.2, pp.331-345, 2012.
DOI : 10.1038/ncb1765

URL : http://jcb.rupress.org/content/jcb/199/2/331.full.pdf

M. C. Weiger, C. C. Wang, M. Krajcovic, A. T. Melvin, J. J. Rhoden et al., Spontaneous phosphoinositide 3-kinase signaling dynamics drive spreading and random migration of fibroblasts, Journal of Cell Science, vol.122, issue.3, pp.313-323, 2009.
DOI : 10.1242/jcs.037564

URL : http://jcs.biologists.org/content/joces/122/3/313.full.pdf

E. Wertheimer, A. Gutierrez-uzquiza, C. Rosemblit, C. Lopez-haber, M. S. Sosa et al., Rac signaling in breast cancer: A tale of GEFs and GAPs, Cellular Signalling, vol.24, issue.2, pp.353-362, 2012.
DOI : 10.1016/j.cellsig.2011.08.011

B. Woods, C. C. Kuo, C. F. Wu, T. R. Zyla, L. et al., Polarity establishment requires localized activation of Cdc42, The Journal of Cell Biology, vol.211, issue.1, pp.19-26, 2015.
DOI : 10.1091/mbc.4.12.1307

URL : http://jcb.rupress.org/content/jcb/211/1/19.full.pdf

H. W. Yang, S. Collins, M. , and T. , Locally excitable Cdc42 signals steer cells during??chemotaxis, Nature Cell Biology, vol.86, issue.2, pp.191-201, 2016.
DOI : 10.1038/nmeth.1201

URL : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015690/pdf

B. Zhang, Y. Gao, S. Y. Moon, Y. Zhang, and Y. Zheng, Oligomerization of Rac1 GTPase Mediated by the Carboxyl-terminal Polybasic Domain, Journal of Biological Chemistry, vol.269, issue.12, pp.8958-8967, 2001.
DOI : 10.1074/jbc.M003780200

Y. Zhou and J. F. Hancock, Ras nanoclusters: Versatile lipid-based signaling platforms, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, vol.1853, issue.4, pp.841-849, 2015.
DOI : 10.1016/j.bbamcr.2014.09.008

URL : https://doi.org/10.1016/j.bbamcr.2014.09.008